综合区系学:分子时代生物多样性调查的现代方法,应用于俄罗斯西伯利亚南部Khamar Daban山脉的探险

IF 1.2 3区 生物学 Q3 PLANT SCIENCES Journal of Bryology Pub Date : 2022-04-03 DOI:10.1080/03736687.2022.2078767
V. Fedosov, O. M. Afonina, M. Ignatov, E. Ignatova, S. G. Kazanovsky, O. I. Kuznetsova, Y. S. Mamontov, N. Konstantinova, Darja E. Koltysheva, S. Kubešová, Michel P. Lamkowski, Alžběta Manukjanová, N. Gamova, A. Fedorova, S. Dudov, A. Verkhozina, J. Kučera
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引用次数: 3

摘要

摘要简介我们报道了对俄罗斯西伯利亚南部的卡马尔-达班山脉进行的国际苔藓学考察的结果。本文介绍了一种新的综合区系学方法,即通过结合形态学和分子证据来识别和验证意外发现,来调查一个地区的区系。我们表明,在可以进行直接分子比较的情况下,或者可以很容易地从为最近的治疗提供信息的可靠研究中获得相关数据,使用这种方法可以提供高水平的可信度。综合区系学也可用于识别需要进一步研究的潜在分类学问题。方法以植物解剖学和形态学为基础的常规鉴定方法,对不完全符合现有形态学概念或根据先前建立的植物地理学模式无法得到记录的标本,辅以分子条形码(细胞核ITS、质体trnL–trnF、rps4和atpB–rbcL区域)。主要结果和结论我们总共记录了来自Khamar Daban山脉北坡五个地区的约380种苔藓和约150种苔类植物。这种区系丰富度表明苔藓植物多样性是一个热点,具有显著高比例的亚海洋物种,如毛状Brachydontium trichodes(F.Weber)Milde、条纹Herzogiella striatella(Brid.)Z.Iwats和Racomitrium macounii亚种。alpinum(E.Lawton)Frisvoll。近20%的标本是用DNA条形码识别或检查的。这种方法表明,在许多属中存在以前未记录的物种,包括Didyodon、Neckera和Pseudohypohypnum。
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Integrative floristics: a modern approach to biodiversity surveys in the molecular era, as applied to an expedition to the Khamar-Daban range, southern Siberia, Russia
ABSTRACT Introduction We report the results of an international bryological expedition to the Khamar-Daban mountain range in Russian southern Siberia. The novel approach of integrative floristics is introduced here as a method of surveying the flora of a region by using a combination of morphological and molecular evidence to identify and verify unexpected findings. We show that use of this approach can deliver a high level of credibility in cases in which direct molecular comparison is possible or the relevant data can be easily obtained from reliable studies informing recent treatments. Integrative floristics can also be used to identify potential taxonomic problems requiring further study. Methods Conventional identification methods based on plant anatomy and morphology were supplemented with molecular barcoding (nuclear ITS, plastid trnL–trnF, rps4 and atpB–rbcL regions) of specimens that either did not fit existing morphological concepts perfectly or for which records were unexpected based on previously established phytogeographical patterns. Key results and conclusions In total, we recorded ca 380 species of mosses and ca 150 species of liverworts from five localities on the northern slopes of the Khamar-Daban range. Such floristic richness indicates a hotspot of bryophyte diversity with a remarkably high proportion of suboceanic species, such as Brachydontium trichodes (F.Weber) Milde, Herzogiella striatella (Brid.) Z.Iwats. and Racomitrium macounii subsp. alpinum (E.Lawton) Frisvoll. Nearly 20% of specimens were identified or checked using DNA barcoding. This approach showed the presence of previously unrecorded species in a number of genera, including Didymodon, Neckera and Pseudohygrohypnum.
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来源期刊
Journal of Bryology
Journal of Bryology 生物-植物科学
CiteScore
4.50
自引率
31.60%
发文量
20
审稿时长
3 months
期刊介绍: Journal of Bryology exists to promote the scientific study of bryophytes (mosses, peat-mosses, liverworts and hornworts) and to foster understanding of the wider aspects of bryology. Journal of Bryology is an international botanical periodical which publishes original research papers in cell biology, anatomy, development, genetics, physiology, chemistry, ecology, paleobotany, evolution, taxonomy, molecular systematics, applied biology, conservation, biomonitoring and biogeography of bryophytes, and also significant new check-lists and descriptive floras of poorly known regions and studies on the role of bryophytes in human affairs, and the lives of notable bryologists.
期刊最新文献
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