成年斑马鱼体内多模态成像专用室。

IF 1.4 4区生物学 Q4 DEVELOPMENTAL BIOLOGY Zebrafish Pub Date : 2022-03-16 DOI:10.1089/zeb.2021.0066

Steven Seeger, M. Zvolsky, Salim Melikov, Maja Frerkes, M. Rafecas

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引用次数: 4

摘要

成年斑马鱼的体内成像具有挑战性，特别是在使用正电子发射断层扫描、单光子发射计算机断层扫描、计算机断层扫描(CT)或磁共振成像(MRI)时进行动态或长时间采集。水环境是确保动物福利必不可少的，但商业小动物成像室不提供这样的条件，因为它们是为啮齿动物设计的。在这项研究中，我们提出了一个专用的流经腔，包括鱼类固定，允许连续供应淡水和麻醉剂，以及清除排泄物。流动模拟和实验，以及MRI和CT的首次扫描，都支持了该腔室的适用性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Dedicated Chamber for Multimodal In Vivo Imaging of Adult Zebrafish.

In vivo imaging of adult zebrafish is challenging, particularly for dynamic or long acquisitions when using, for example, positron emission tomography, single photon emission computed tomography, computed tomography (CT), or magnetic resonance imaging (MRI). An aqueous environment is indispensable to ensure animal welfare, but commercial small-animal imaging chambers do not provide such conditions, as they are designed for rodents. In this study we present a dedicated flow-through chamber that includes fish immobilization and allows for the continuous supply of fresh water and anesthetics, as well as the removal of excretions. Both flow simulations and experiments, as well as first scans with MRI and CT, support the suitability of the chamber.

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来源期刊

Zebrafish DEVELOPMENTAL BIOLOGY-ZOOLOGY

CiteScore

3.60

自引率

5.00%

发文量

审稿时长

3 months

期刊介绍： Zebrafish is the only peer-reviewed journal dedicated to the central role of zebrafish and other aquarium species as models for the study of vertebrate development, evolution, toxicology, and human disease. Due to its prolific reproduction and the external development of the transparent embryo, the zebrafish is a prime model for genetic and developmental studies. While genetically more distant from humans, the vertebrate zebrafish nevertheless has comparable organs and tissues, such as heart, kidney, pancreas, bones, and cartilage. Zebrafish introduced the new section TechnoFish, which highlights these innovations for the general zebrafish community. TechnoFish features two types of articles: TechnoFish Previews: Important, generally useful technical advances or valuable transgenic lines TechnoFish Methods: Brief descriptions of new methods, reagents, or transgenic lines that will be of widespread use in the zebrafish community Zebrafish coverage includes: Comparative genomics and evolution Molecular/cellular mechanisms of cell growth Genetic analysis of embryogenesis and disease Toxicological and infectious disease models Models for neurological disorders and aging New methods, tools, and experimental approaches Zebrafish also includes research with other aquarium species such as medaka, Fugu, and Xiphophorus.