Agronomic Zn biofortification through nano ZnO application enhanced growth, photosystem efficiency, Zn and P nutrition in maize

ABSTRACT Zinc-oxide nanoparticles (ZnO-NP) effect on crop physiology and zinc recovery remains poorly studied for acidic-sandy soils. To address this, greenhouse pot (plastic-pots, 6 kg soil, maize) experiments with ZnO-NP (50, 100, 150, 200 mg kg−1) applied via different methods (soil-drench, seed-coating and foliar-spray) was conducted in a 60 days study. Results revealed that ZnO-NP via seed-coating (100 mg kg−1) and soil-drench (150 mg kg−1) enhanced shoot and total P uptake, while ZnO-NPs (foliar) (50 mg kg−1) enhanced maize growth (6–11%), with agronomic and physiological improvements ultimately resulted in greater biomass (16–20%), Zn agronomic efficiency and uptake. Compared to ZnSO4 treatment and the control, seed-coating with 100 mg kg−1 ZnO-NP increased leaf chlorophyll and pigment content by 12–127%. Principal component analysis revealed a close association among growth traits, plant pigments, fluorescence parameters, total Zn and P concentration, and uptake with total biomass as influenced by ZnO-NPs. Thus, compared to conventional ZnSO4 and higher dosages of ZnO-NPs, foliar-spray of ZnO-NP at 50 mg kg−1, seed-coating at 100 mg kg−1, or soil-drench at 150 mg kg−1 increased maize biochemical characteristics, growth, biomass, and Zn agronomic efficiency. These elucidate important implications of ZnO-NP application for increasing plant development and Zn biofortification in acidic-sandy soils.