{"title":"长非编码RNA母系表达基因3通过Wnt/人连环蛋白信号通路对胶质瘤细胞增殖和侵袭能力的影响","authors":"Shifeng Yang, Lei Wang, Xiao Ma, Mingqi Yang","doi":"10.3760/CMA.J.ISSN.1001-9030.2020.01.019","DOIUrl":null,"url":null,"abstract":"Objective \nTo investigate the effect of long non-coding RNA maternally expressed gene 3 (MEG3) on the proliferation and invasion capacity of glioma cells through the Wnt/human-catenin signal pathway. \n \n \nMethods \nGlioma cells were divided into 3 groups: the blank control group (CON), the gene transfection group (MEG3) and the gene inhibition group (siMEG3). The cells in blank group were left untreated and cultured normally. MEG3 gene was transfected in the gene transfection group, and gene interference was conducted in the gene inhibition group. Protein expression was determined by Western blotting, and gene expression was detected by real-time quantitative reverse transcriptase-polymerase chain reaction (RT-qPCR). \n \n \nResults \nAfter transfection of MEG3 gene, the apoptosis rate increased to 61.05±2.77 with the prolongation of culture time, which was higher than that of the control group and the gene inhibition group (F=23.543, P<0.05). After MEG3 gene knockout, the apoptosis rate of glioma cells was higher than that of the control group (P<0.05). After 48 hours of transfection, the number of cell migration was 105.36±15.27 (F=33.350, P<0.05). When MEG3 gene was inhibited, the number of cell migration was 989.41±11.06, higher than that of the control group (F=40.667, P<0.05). After MEG3 gene transfection, the number of invasion cells was 251.25±35.85, which was lower than that of the control group and gene knockout group (F=31.167, P<0.05). After MEG3 gene was knocked out, the number of invasion cells was 1 500.00± 84.76, which was higher than that of the blank control group (F=63.762, P<0.05). Compared with the gene inhibition group, the c-Jun gene of the control group and the gene transfection group decreased, and the gene transfection group was lower than the blank control group (F=15.426, P<0.05). \n \n \nConclusion \nLong non-coding RNA MEG3 can inhibit the proliferation and invasion of glioma cells through the Wnt/human-catenin signal pathway. \n \n \nKey words: \nGlioma; Protein; Gene","PeriodicalId":10065,"journal":{"name":"中华实验外科杂志","volume":"37 1","pages":"67-70"},"PeriodicalIF":0.0000,"publicationDate":"2020-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effect of long non-coding RNA maternally expressed gene 3 on proliferation and invasion capacity of glioma cells through the Wnt/human-catenin signal pathway\",\"authors\":\"Shifeng Yang, Lei Wang, Xiao Ma, Mingqi Yang\",\"doi\":\"10.3760/CMA.J.ISSN.1001-9030.2020.01.019\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Objective \\nTo investigate the effect of long non-coding RNA maternally expressed gene 3 (MEG3) on the proliferation and invasion capacity of glioma cells through the Wnt/human-catenin signal pathway. \\n \\n \\nMethods \\nGlioma cells were divided into 3 groups: the blank control group (CON), the gene transfection group (MEG3) and the gene inhibition group (siMEG3). The cells in blank group were left untreated and cultured normally. MEG3 gene was transfected in the gene transfection group, and gene interference was conducted in the gene inhibition group. Protein expression was determined by Western blotting, and gene expression was detected by real-time quantitative reverse transcriptase-polymerase chain reaction (RT-qPCR). \\n \\n \\nResults \\nAfter transfection of MEG3 gene, the apoptosis rate increased to 61.05±2.77 with the prolongation of culture time, which was higher than that of the control group and the gene inhibition group (F=23.543, P<0.05). After MEG3 gene knockout, the apoptosis rate of glioma cells was higher than that of the control group (P<0.05). After 48 hours of transfection, the number of cell migration was 105.36±15.27 (F=33.350, P<0.05). When MEG3 gene was inhibited, the number of cell migration was 989.41±11.06, higher than that of the control group (F=40.667, P<0.05). After MEG3 gene transfection, the number of invasion cells was 251.25±35.85, which was lower than that of the control group and gene knockout group (F=31.167, P<0.05). After MEG3 gene was knocked out, the number of invasion cells was 1 500.00± 84.76, which was higher than that of the blank control group (F=63.762, P<0.05). Compared with the gene inhibition group, the c-Jun gene of the control group and the gene transfection group decreased, and the gene transfection group was lower than the blank control group (F=15.426, P<0.05). \\n \\n \\nConclusion \\nLong non-coding RNA MEG3 can inhibit the proliferation and invasion of glioma cells through the Wnt/human-catenin signal pathway. \\n \\n \\nKey words: \\nGlioma; Protein; Gene\",\"PeriodicalId\":10065,\"journal\":{\"name\":\"中华实验外科杂志\",\"volume\":\"37 1\",\"pages\":\"67-70\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2020-01-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"中华实验外科杂志\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.3760/CMA.J.ISSN.1001-9030.2020.01.019\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"中华实验外科杂志","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3760/CMA.J.ISSN.1001-9030.2020.01.019","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Effect of long non-coding RNA maternally expressed gene 3 on proliferation and invasion capacity of glioma cells through the Wnt/human-catenin signal pathway
Objective
To investigate the effect of long non-coding RNA maternally expressed gene 3 (MEG3) on the proliferation and invasion capacity of glioma cells through the Wnt/human-catenin signal pathway.
Methods
Glioma cells were divided into 3 groups: the blank control group (CON), the gene transfection group (MEG3) and the gene inhibition group (siMEG3). The cells in blank group were left untreated and cultured normally. MEG3 gene was transfected in the gene transfection group, and gene interference was conducted in the gene inhibition group. Protein expression was determined by Western blotting, and gene expression was detected by real-time quantitative reverse transcriptase-polymerase chain reaction (RT-qPCR).
Results
After transfection of MEG3 gene, the apoptosis rate increased to 61.05±2.77 with the prolongation of culture time, which was higher than that of the control group and the gene inhibition group (F=23.543, P<0.05). After MEG3 gene knockout, the apoptosis rate of glioma cells was higher than that of the control group (P<0.05). After 48 hours of transfection, the number of cell migration was 105.36±15.27 (F=33.350, P<0.05). When MEG3 gene was inhibited, the number of cell migration was 989.41±11.06, higher than that of the control group (F=40.667, P<0.05). After MEG3 gene transfection, the number of invasion cells was 251.25±35.85, which was lower than that of the control group and gene knockout group (F=31.167, P<0.05). After MEG3 gene was knocked out, the number of invasion cells was 1 500.00± 84.76, which was higher than that of the blank control group (F=63.762, P<0.05). Compared with the gene inhibition group, the c-Jun gene of the control group and the gene transfection group decreased, and the gene transfection group was lower than the blank control group (F=15.426, P<0.05).
Conclusion
Long non-coding RNA MEG3 can inhibit the proliferation and invasion of glioma cells through the Wnt/human-catenin signal pathway.
Key words:
Glioma; Protein; Gene
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