B. Heredia-Mercado, F. Delgado-Vargas, L. U. Osuna-Martínez, E. Noguera-Corona, J. López-Valenzuela, R. Ramos-Payán, G. López-Angulo
{"title":"马尾莲叶水醇提取物的抗糖尿病作用及慢性毒性研究","authors":"B. Heredia-Mercado, F. Delgado-Vargas, L. U. Osuna-Martínez, E. Noguera-Corona, J. López-Valenzuela, R. Ramos-Payán, G. López-Angulo","doi":"10.5530/pres.15.2.034","DOIUrl":null,"url":null,"abstract":"Background: Echeveria subrigida extracts have biological activities of human health importance. However, the in vivo effects on glucose levels and chronic toxicity are unknown. Objectives: To analyse the in vivo hypoglycemic and antihyperglycemic effects (50, 100, and 200 mg/kg b.w.) and chronic toxicity (1000 mg/kg b.w. for 270 days) of the hydroalcoholic extract of E. subrigida (HE–Es) in BALB/c mice. Materials and Methods: The HE–Es was analysed by HPLC. Glucose levels were measured to establish the effects on glycemia. Different parameters were registered in the toxicity assay: e.g. , feed consumption, appearance/behaviour, biochemical and haematological parameters and liver and kidney histologies. Results: Quercetin–3-O -β-glucoside and isorhamnetin–3-O -β-glucoside were the main flavonoids in the HE–Es. Glycemia was reduced by the HE–Es (200 mg/kg b.w., 49.1%) and glibenclamide (10 mg/kg b.w., 52%) treatments. Comparing the antihyperglycemic activities, similar reductions were found between HE–Es (100 mg/kg b.w., 29.32%; 200 mg/kg, 28.99%) and acarbose (10 mg/kg b.w., 19.87%) treatments. On the other hand, the HE–Es was innocuous in mice (LD 50 > 1000 mg/kg b.w.), and the results suggested that HE–Es had adaptogenic, and immunostimulant activities. The hepatic and renal histologies were normal; however, male mice showed level zero steatosis that disappeared in the HE–Es satellite after treatment withdrawal. Conclusion: This study reports for the first time the in vivo effects on blood glucose levels of HE–Es and chronic toxicity of a Crassulaceae plant, supporting the antidiabetic potential and safety of HE–Es. Future studies must corroborate the effects of HE–Es in humans, allowing its use in high-value formulations.","PeriodicalId":19813,"journal":{"name":"Pharmacognosy Research","volume":" ","pages":""},"PeriodicalIF":0.7000,"publicationDate":"2023-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Antidiabetic Potential and Chronic Toxicity of Hydroalcoholic Extract of Echeveria subrigida Leaves\",\"authors\":\"B. Heredia-Mercado, F. Delgado-Vargas, L. U. Osuna-Martínez, E. Noguera-Corona, J. López-Valenzuela, R. Ramos-Payán, G. López-Angulo\",\"doi\":\"10.5530/pres.15.2.034\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Background: Echeveria subrigida extracts have biological activities of human health importance. However, the in vivo effects on glucose levels and chronic toxicity are unknown. Objectives: To analyse the in vivo hypoglycemic and antihyperglycemic effects (50, 100, and 200 mg/kg b.w.) and chronic toxicity (1000 mg/kg b.w. for 270 days) of the hydroalcoholic extract of E. subrigida (HE–Es) in BALB/c mice. Materials and Methods: The HE–Es was analysed by HPLC. Glucose levels were measured to establish the effects on glycemia. Different parameters were registered in the toxicity assay: e.g. , feed consumption, appearance/behaviour, biochemical and haematological parameters and liver and kidney histologies. Results: Quercetin–3-O -β-glucoside and isorhamnetin–3-O -β-glucoside were the main flavonoids in the HE–Es. Glycemia was reduced by the HE–Es (200 mg/kg b.w., 49.1%) and glibenclamide (10 mg/kg b.w., 52%) treatments. Comparing the antihyperglycemic activities, similar reductions were found between HE–Es (100 mg/kg b.w., 29.32%; 200 mg/kg, 28.99%) and acarbose (10 mg/kg b.w., 19.87%) treatments. On the other hand, the HE–Es was innocuous in mice (LD 50 > 1000 mg/kg b.w.), and the results suggested that HE–Es had adaptogenic, and immunostimulant activities. The hepatic and renal histologies were normal; however, male mice showed level zero steatosis that disappeared in the HE–Es satellite after treatment withdrawal. Conclusion: This study reports for the first time the in vivo effects on blood glucose levels of HE–Es and chronic toxicity of a Crassulaceae plant, supporting the antidiabetic potential and safety of HE–Es. Future studies must corroborate the effects of HE–Es in humans, allowing its use in high-value formulations.\",\"PeriodicalId\":19813,\"journal\":{\"name\":\"Pharmacognosy Research\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":0.7000,\"publicationDate\":\"2023-02-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Pharmacognosy Research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.5530/pres.15.2.034\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"PHARMACOLOGY & PHARMACY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pharmacognosy Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5530/pres.15.2.034","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PHARMACOLOGY & PHARMACY","Score":null,"Total":0}
Antidiabetic Potential and Chronic Toxicity of Hydroalcoholic Extract of Echeveria subrigida Leaves
Background: Echeveria subrigida extracts have biological activities of human health importance. However, the in vivo effects on glucose levels and chronic toxicity are unknown. Objectives: To analyse the in vivo hypoglycemic and antihyperglycemic effects (50, 100, and 200 mg/kg b.w.) and chronic toxicity (1000 mg/kg b.w. for 270 days) of the hydroalcoholic extract of E. subrigida (HE–Es) in BALB/c mice. Materials and Methods: The HE–Es was analysed by HPLC. Glucose levels were measured to establish the effects on glycemia. Different parameters were registered in the toxicity assay: e.g. , feed consumption, appearance/behaviour, biochemical and haematological parameters and liver and kidney histologies. Results: Quercetin–3-O -β-glucoside and isorhamnetin–3-O -β-glucoside were the main flavonoids in the HE–Es. Glycemia was reduced by the HE–Es (200 mg/kg b.w., 49.1%) and glibenclamide (10 mg/kg b.w., 52%) treatments. Comparing the antihyperglycemic activities, similar reductions were found between HE–Es (100 mg/kg b.w., 29.32%; 200 mg/kg, 28.99%) and acarbose (10 mg/kg b.w., 19.87%) treatments. On the other hand, the HE–Es was innocuous in mice (LD 50 > 1000 mg/kg b.w.), and the results suggested that HE–Es had adaptogenic, and immunostimulant activities. The hepatic and renal histologies were normal; however, male mice showed level zero steatosis that disappeared in the HE–Es satellite after treatment withdrawal. Conclusion: This study reports for the first time the in vivo effects on blood glucose levels of HE–Es and chronic toxicity of a Crassulaceae plant, supporting the antidiabetic potential and safety of HE–Es. Future studies must corroborate the effects of HE–Es in humans, allowing its use in high-value formulations.
期刊介绍:
Pharmacognosy Research [ISSN: Print -0976-4836, Online - 0974-8490] [http://www.phcogres.com], Quarterly a publication of Phcog.Net is published by Wolters Kluwer - Medknow Publications. It provides peer-reviewed original research articles from the field of Natural Products. The journal serves an international audience of scientists and researchers in a variety of research and academia by quickly disseminating research findings related to Medicinal Plants and Natural Products. It is a peer reviewed journal aiming to publish high quality original research articles, methods, techniques and evaluation reports, critical reviews, short communications, commentaries and editorials of all aspects of medicinal plant research. The journal is aimed at a broad readership, publishing articles on all aspects of pharmacognosy, and related fields. The journal aims to increase understanding of pharmacognosy as well as to direct and foster further research through the dissemination of scientific information by the publication of manuscripts. The submissions of original contributions in all areas of pharmacognosy are welcome.