基于质谱的结构蛋白组学研究进展:从重组蛋白到细胞系统的HDX‐MS和XL‐MS技术的发展

IF 2.3 4区 化学 Q3 CHEMISTRY, MULTIDISCIPLINARY Israel Journal of Chemistry Pub Date : 2023-08-07 DOI:10.1002/ijch.202300084
Valentine V. Courouble, Matthew D. Mann, Patrick R. Griffin
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引用次数: 0

摘要

质谱(MS)是一种用于研究蛋白质和生物分子的核心分析技术。它测量离子的质量电荷比,以识别和量化简单和复杂混合物中的分子。仪器、样品制备方法和数据分析工作流程方面的技术进步继续推动质谱的能力,以回答更复杂的问题,反之亦然。结构蛋白质组学使用基于质谱的方法来表征蛋白质结构。具体来说,但不限于,氢氘交换质谱(HDX‐MS)和交联质谱(XL‐MS)是互补的技术,可以捕获溶液中蛋白质固有的结构可塑性。本综述旨在介绍HDX - MS和XL - MS的最新进展,这些进展使这些技术不仅可以用于简单的重组蛋白系统,还可以用于复杂的细胞系统。
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Advances in Mass Spectrometry-Based Structural Proteomics: Development of HDX-MS and XL-MS Techniques from Recombinant Protein to Cellular Systems

Mass spectrometry (MS) is a central analytical technique used to study proteins and biomolecules. It measures mass-to-charge ratio of ions to identify and quantify molecules in simple and complex mixtures. Technological advancement in instrumentation, sample preparation methodologies, and data analysis workflows continue to push the capabilities of MS to answer more complicated questions and vice versa. Structural proteomics uses MS-based methodologies to characterize protein structure. Specifically, but not limited to, hydrogen deuterium exchange MS (HDX-MS) and crosslinking MS (XL-MS) are complementary techniques that capture the structural plasticity inherent to proteins in solution. This review is intended to present recent progress in HDX-MS and XL-MS that have allowed these techniques to be used not only for simple recombinant protein systems but with complex cellular systems.

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来源期刊
Israel Journal of Chemistry
Israel Journal of Chemistry 化学-化学综合
CiteScore
6.20
自引率
0.00%
发文量
62
审稿时长
6-12 weeks
期刊介绍: The fledgling State of Israel began to publish its scientific activity in 1951 under the general heading of Bulletin of the Research Council of Israel, which quickly split into sections to accommodate various fields in the growing academic community. In 1963, the Bulletin ceased publication and independent journals were born, with Section A becoming the new Israel Journal of Chemistry. The Israel Journal of Chemistry is the official journal of the Israel Chemical Society. Effective from Volume 50 (2010) it is published by Wiley-VCH. The Israel Journal of Chemistry is an international and peer-reviewed publication forum for Special Issues on timely research topics in all fields of chemistry: from biochemistry through organic and inorganic chemistry to polymer, physical and theoretical chemistry, including all interdisciplinary topics. Each topical issue is edited by one or several Guest Editors and primarily contains invited Review articles. Communications and Full Papers may be published occasionally, if they fit with the quality standards of the journal. The publication language is English and the journal is published twelve times a year.
期刊最新文献
Cover Picture: (Isr. J. Chem. 8-9/2024) Special Issue on RNA-Based Catalysts that Revolutionized the Discovery of Bioactive Peptides Hexagonal and Trigonal Quasiperiodic Tilings Breaking the Degeneracy of Sense Codons – How Far Can We Go? Cover Picture: (Isr. J. Chem. 6-7/2024)
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