L. D. Saputri, S. Isminingsih, A. A. Fatmawaty, F. Rachmawati
{"title":"台湾金线莲离体生殖","authors":"L. D. Saputri, S. Isminingsih, A. A. Fatmawaty, F. Rachmawati","doi":"10.33512/JUR.AGROEKOTEK.V11I1.7620","DOIUrl":null,"url":null,"abstract":"ABSTRACT Anoectochilus formosanus is one species of Orchids, known as a “Jewel Orchids” and have been used as a folk medicine in China and Taiwan.The aim of this study was to examine the response of using different types of explants and combination of growth regulators TDZ and BAP to morphogenesis of Anoectochilus formosanus Orchids by in vitro. The results showed that using different types of explants had very significant effect to the percentage of callus and shoot morphogenesis on 12 weeks after planting, the number of adventitious buds, adventitious shoot length, and callus diameter on three weeks until 12 weeks after planting. The best explant to callus morphogenesis was showing on shoot tip explant (50%) and to shoot morphogenesis was showing on auxillary buds and internode explant (100%). The best number of adventitious buds was observed in second internode explant with average number of shoots are 7.62 shoots. The best adventititous length was observed in axillary buds explant with average number of shoot length in 1.16 cm, and the best callus diameter was observed in shoot tip explant with average of diameter in 2.2 mm. The combination of plant growth regulator TDZ and BAP had a very significant effect to adventitious length on 10 weeks after planting, and a significant effect to adventitious length on 11 and 12 weeks after planting. The best adventitious length was observed on 0.25 mg L -1 TDZ + 0.75 mg L -1 BAP in 1.13 cm per explant on 12 weeks after planting. The best combination to callus and shoot morphogenesis was observed on 0.75 mg L -1 TDZ + 0.25 mg L -1 BAP. 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引用次数: 0
摘要
摘要台湾凤仙兰(Anoectochilus formosanus)是兰花的一种,被称为“宝石兰花”,在中国大陆和台湾都被用作民间药物。本研究旨在探讨不同外植体及生长调节剂TDZ和BAP对台湾凤尾兰体外形态发生的影响。结果表明:不同外植体对植后12周的愈伤组织百分率和芽形态发生、植后3周至12周的不定芽数、不定芽长和愈伤组织直径均有极显著影响。愈伤组织形态发生的最佳外植体为茎尖外植体(50%),茎部形态发生的最佳外植体为副芽和节间外植体(100%)。第二节间外植体不定芽数量最多,平均芽数为7.62个。腋芽外植体的不定长最佳,平均茎长为1.16 cm;茎尖外植体的愈伤组织直径最佳,平均茎长为2.2 mm。植物生长调节剂TDZ和BAP在植后10周对不定长有极显著影响,在植后11周和12周对不定长有显著影响。种植12周后,0.25 mg L -1 TDZ + 0.75 mg L -1 BAP的不定长为1.13 cm /外植体。0.75 mg L -1 TDZ + 0.25 mg L -1 BAP处理对愈伤组织和芽部形态发生的影响最大。使用不同类型外植体与组合植物生长调节剂对台湾凤仙花的体外形态发生无交互作用。
MORFOGENESIS ANGGREK (Anoectochilus formosanus) SECARA IN VITRO
ABSTRACT Anoectochilus formosanus is one species of Orchids, known as a “Jewel Orchids” and have been used as a folk medicine in China and Taiwan.The aim of this study was to examine the response of using different types of explants and combination of growth regulators TDZ and BAP to morphogenesis of Anoectochilus formosanus Orchids by in vitro. The results showed that using different types of explants had very significant effect to the percentage of callus and shoot morphogenesis on 12 weeks after planting, the number of adventitious buds, adventitious shoot length, and callus diameter on three weeks until 12 weeks after planting. The best explant to callus morphogenesis was showing on shoot tip explant (50%) and to shoot morphogenesis was showing on auxillary buds and internode explant (100%). The best number of adventitious buds was observed in second internode explant with average number of shoots are 7.62 shoots. The best adventititous length was observed in axillary buds explant with average number of shoot length in 1.16 cm, and the best callus diameter was observed in shoot tip explant with average of diameter in 2.2 mm. The combination of plant growth regulator TDZ and BAP had a very significant effect to adventitious length on 10 weeks after planting, and a significant effect to adventitious length on 11 and 12 weeks after planting. The best adventitious length was observed on 0.25 mg L -1 TDZ + 0.75 mg L -1 BAP in 1.13 cm per explant on 12 weeks after planting. The best combination to callus and shoot morphogenesis was observed on 0.75 mg L -1 TDZ + 0.25 mg L -1 BAP. There was no interaction between used a different types of explant and combination plant growth regulator to morphogenesis Anoectochilus formosanus orchids by in vitro.