{"title":"甜菊叶直接芽增殖技术在健康生物质生产中的应用","authors":"Ahmed Saad Attaya","doi":"10.21608/AGRO.2021.66749.1252","DOIUrl":null,"url":null,"abstract":"AN EFFICIENT and simple regeneration protocol using leaf explants was described on MS medium in the presence of various plant growth regulators. After four weeks of culture, the highest induction of adventitious shoot buds from leaf culture (90%) was achieved on MS medium with 1.0mg/L BA. The largest number of shoot buds (3.02) per leaf explant with no callus formation was achieved on MS medium with 2.0mg/L BA. The best combination for the induction of numerous shoot buds was found to be TDZ + BA + IBA (0.5, 1.0, and 0.5mg/L, respectively), which presented 10.4 shoots per explant with 3.15cm in length. For in vitro root formation stage, Healthy shoots were collected and cultivated on a half-strength MS with various concentrations of NAA and IAA alone or in combination with 2,4-D or IBA. Furthermore, the highest roots (8.17) per shoot with root formation (100 %) was observed using a half-strength MS medium in the presence of 2.0mg/L IAA. Therefore, healthy in vitro rooted plantlets were acclimatized successfully after four weeks in the greenhouse.","PeriodicalId":42226,"journal":{"name":"Egyptian Journal of Agronomy","volume":null,"pages":null},"PeriodicalIF":0.5000,"publicationDate":"2021-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Direct Shoot Bud Proliferation Protocol from Stevia rebaudiana Leaf Culture for Healthy Biomass Production\",\"authors\":\"Ahmed Saad Attaya\",\"doi\":\"10.21608/AGRO.2021.66749.1252\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"AN EFFICIENT and simple regeneration protocol using leaf explants was described on MS medium in the presence of various plant growth regulators. After four weeks of culture, the highest induction of adventitious shoot buds from leaf culture (90%) was achieved on MS medium with 1.0mg/L BA. The largest number of shoot buds (3.02) per leaf explant with no callus formation was achieved on MS medium with 2.0mg/L BA. The best combination for the induction of numerous shoot buds was found to be TDZ + BA + IBA (0.5, 1.0, and 0.5mg/L, respectively), which presented 10.4 shoots per explant with 3.15cm in length. For in vitro root formation stage, Healthy shoots were collected and cultivated on a half-strength MS with various concentrations of NAA and IAA alone or in combination with 2,4-D or IBA. Furthermore, the highest roots (8.17) per shoot with root formation (100 %) was observed using a half-strength MS medium in the presence of 2.0mg/L IAA. Therefore, healthy in vitro rooted plantlets were acclimatized successfully after four weeks in the greenhouse.\",\"PeriodicalId\":42226,\"journal\":{\"name\":\"Egyptian Journal of Agronomy\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.5000,\"publicationDate\":\"2021-04-21\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Egyptian Journal of Agronomy\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.21608/AGRO.2021.66749.1252\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"AGRONOMY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Egyptian Journal of Agronomy","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.21608/AGRO.2021.66749.1252","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"AGRONOMY","Score":null,"Total":0}
引用次数: 0
摘要
在多种植物生长调节剂的作用下,利用叶片外植体在MS培养基上进行高效、简便的再生。培养4周后,在添加1.0mg/L BA的MS培养基上,叶片不定芽的诱导率最高(90%)。在BA浓度为2.0mg/L的MS培养基上,叶片外植体的芽数最多,为3.02个,且无愈伤组织形成。TDZ + BA + IBA(分别为0.5、1.0和0.5mg/L)是诱导芽数最多的最佳组合,每个外植体可产生10.4个芽,芽长3.15cm。在离体根形成阶段,采集健康苗,在不同浓度NAA和IAA单独或联合2,4- d或IBA的半强MS培养基上培养。在2.0mg/L IAA浓度为半强度的MS培养基中,每枝生根数最高,为8.17根,生根率为100%。因此,健康的离体生根植株在温室中培养4周后就能成功地适应环境。
Direct Shoot Bud Proliferation Protocol from Stevia rebaudiana Leaf Culture for Healthy Biomass Production
AN EFFICIENT and simple regeneration protocol using leaf explants was described on MS medium in the presence of various plant growth regulators. After four weeks of culture, the highest induction of adventitious shoot buds from leaf culture (90%) was achieved on MS medium with 1.0mg/L BA. The largest number of shoot buds (3.02) per leaf explant with no callus formation was achieved on MS medium with 2.0mg/L BA. The best combination for the induction of numerous shoot buds was found to be TDZ + BA + IBA (0.5, 1.0, and 0.5mg/L, respectively), which presented 10.4 shoots per explant with 3.15cm in length. For in vitro root formation stage, Healthy shoots were collected and cultivated on a half-strength MS with various concentrations of NAA and IAA alone or in combination with 2,4-D or IBA. Furthermore, the highest roots (8.17) per shoot with root formation (100 %) was observed using a half-strength MS medium in the presence of 2.0mg/L IAA. Therefore, healthy in vitro rooted plantlets were acclimatized successfully after four weeks in the greenhouse.