María Magdalena Ricciardi, Henry S. Smith, Z. Viloria, E. Dixon, D. Szarka, Bob Pearce, R. Villanueva, N. Gauthier
{"title":"美国肯塔基州大麻赤藓（Aculops cannabicola Farkas）昆虫病原真菌汤普森Hirsutella thompsonii的首次报道","authors":"María Magdalena Ricciardi, Henry S. Smith, Z. Viloria, E. Dixon, D. Szarka, Bob Pearce, R. Villanueva, N. Gauthier","doi":"10.1094/php-04-23-0035-br","DOIUrl":null,"url":null,"abstract":"The hemp russet mite (HRM) is a damaging pest of indoor/greenhouse hemp (Cannabis sativa L.) with limited tools for management. In 2021, HRM was identified in University of Kentucky hemp field trials. Large numbers of darkened, dead HRM were observed on leaves and peduncles. Samples of the HRM were disinfested and plated on potato dextrose agar (PDA). Fungal identity was confirmed by amplifying the internal transcribed spacer of the rDNA followed by Sanger sequencing. The isolated fungus was identified as Hirsutella thompsonii with 100% identity. Pathogenicity was validated using detached leaves infested with healthy HRM. Only HRM from the H. thompsonii-inoculated treatment developed fungal growth, while HRM from the controls did not. This study confirmed the pathogenicity of H. thompsonii on HRM. Further research is warranted to improve our understanding of the potential use and efficacy of H. thompsonii as a sustainable management tool.","PeriodicalId":20251,"journal":{"name":"Plant Health Progress","volume":null,"pages":null},"PeriodicalIF":1.7000,"publicationDate":"2023-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"First report of Hirsutella thompsonii as an entomopathogenic fungus of hemp russet mite (Aculops cannabicola Farkas) in Kentucky\",\"authors\":\"María Magdalena Ricciardi, Henry S. Smith, Z. Viloria, E. Dixon, D. Szarka, Bob Pearce, R. Villanueva, N. Gauthier\",\"doi\":\"10.1094/php-04-23-0035-br\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The hemp russet mite (HRM) is a damaging pest of indoor/greenhouse hemp (Cannabis sativa L.) with limited tools for management. In 2021, HRM was identified in University of Kentucky hemp field trials. Large numbers of darkened, dead HRM were observed on leaves and peduncles. Samples of the HRM were disinfested and plated on potato dextrose agar (PDA). Fungal identity was confirmed by amplifying the internal transcribed spacer of the rDNA followed by Sanger sequencing. The isolated fungus was identified as Hirsutella thompsonii with 100% identity. Pathogenicity was validated using detached leaves infested with healthy HRM. Only HRM from the H. thompsonii-inoculated treatment developed fungal growth, while HRM from the controls did not. This study confirmed the pathogenicity of H. thompsonii on HRM. Further research is warranted to improve our understanding of the potential use and efficacy of H. thompsonii as a sustainable management tool.\",\"PeriodicalId\":20251,\"journal\":{\"name\":\"Plant Health Progress\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.7000,\"publicationDate\":\"2023-08-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plant Health Progress\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1094/php-04-23-0035-br\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Health Progress","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1094/php-04-23-0035-br","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
First report of Hirsutella thompsonii as an entomopathogenic fungus of hemp russet mite (Aculops cannabicola Farkas) in Kentucky
The hemp russet mite (HRM) is a damaging pest of indoor/greenhouse hemp (Cannabis sativa L.) with limited tools for management. In 2021, HRM was identified in University of Kentucky hemp field trials. Large numbers of darkened, dead HRM were observed on leaves and peduncles. Samples of the HRM were disinfested and plated on potato dextrose agar (PDA). Fungal identity was confirmed by amplifying the internal transcribed spacer of the rDNA followed by Sanger sequencing. The isolated fungus was identified as Hirsutella thompsonii with 100% identity. Pathogenicity was validated using detached leaves infested with healthy HRM. Only HRM from the H. thompsonii-inoculated treatment developed fungal growth, while HRM from the controls did not. This study confirmed the pathogenicity of H. thompsonii on HRM. Further research is warranted to improve our understanding of the potential use and efficacy of H. thompsonii as a sustainable management tool.
期刊介绍：
Plant Health Progress, a member journal of the Plant Management Network, is a multidisciplinary science-based journal covering all aspects of applied plant health management in agriculture and horticulture. Both peer-reviewed and fully citable, the journal is a credible online-only publication. Plant Health Progress is a not-for-profit collaborative endeavor of the plant health community at large, serving practitioners worldwide. Its primary goal is to provide a comprehensive one-stop Internet resource for plant health information.
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