Virulence gene expression of Pseudomonas aeruginosa isolated from children with nephrotic syndrome

Pseudomonas aeruginosa is Gram-negative, considered as the most common opportunistic pathogens which lead to high rate of mortality and morbidity when subject suffered from compromised immune systems. This study was aimed to estimate the lasI and lasR genes expression of P. aeruginosa isolated from children with nephrotic syndrome. Nineteen isolates from urine samples of children with nephropathy syndrome are included in this study and confirmed by VITEK test as a P. aeruginosa strains. The result showed that the LasI and LasR genes showed a high level of gene expression in strains that grown with ceftriaxone than the strains that grown alone, but the gene Las I had a higher level of expression than the Las R gene, also a regression analysis were done to reveal the effect of Las I on the Las R gene which showed a positive effect which mean any increment in the gene expression level of Las I gene will combined with an increment in the Las R gene, (R= 0.899 and P= 0.001).