Characterization and Bioactivities of Sequentially-Prepared Sea Cucumber Ethanolic Extracts and Protein Hydrolysates

ABSTRACT The sea cucumber ethanol extracts (SCEEs) and protein hydrolysates (SCPHs) of Holothuria atra(HA), H. leucospilota(HL), and Bohadschia marmorata (BM) were prepared sequentially using 60% ethanol and Neutrase. The SCEE of HL contained phenolic acids (4.63 ± 0.50 µg GAE/mg extract) and was the most potent radical scavenger (91.76 ± 0.76%) at 10 mg/mL; whereas its SCPH contained hydrophobic amino acids (31.25 g/100 g) and inhibited glycation by 86.16 ± 5.92% at 5 mg/mL. Furthermore, the SCEE of HA inhibited tyrosinase by 49.93 ± 0.27% at 10 mg/mL, related to sulfated saponins (121.82 ± 3.52 µg xylose/mg extract). This study reports SCEEs as antityrosinase and SCPHs as antiglycation agents for the first time.