石重菌通过抗氧化和抗炎作用对脂多糖诱导的C2C12肌管的抗萎缩作用

IF 2.5 4区 综合性期刊 Q2 CHEMISTRY, MULTIDISCIPLINARY Applied Sciences-Basel Pub Date : 2023-09-08 DOI:10.3390/app131810115
Mi-Bo Kim, Hyeju Lee, Chaehyeon Lee, Yuqing Tan, Sang Gil Lee
{"title":"石重菌通过抗氧化和抗炎作用对脂多糖诱导的C2C12肌管的抗萎缩作用","authors":"Mi-Bo Kim, Hyeju Lee, Chaehyeon Lee, Yuqing Tan, Sang Gil Lee","doi":"10.3390/app131810115","DOIUrl":null,"url":null,"abstract":"Inflammation and oxidative stress are known to be major factors in muscle atrophy. The objective of this study was to evaluate whether the antioxidant activity of Ishige sinicola ethanol extract (ISE) and fractions from ISE could prevent lipopolysaccharide (LPS)-induced muscle atrophy in C2C12 myotubes. IS was extracted with ethanol and fractionated with five organic solvents. Then, ISE and five fractions from ISE were used to evaluate the total antioxidant activity and the protective effect of LPS-induced muscle atrophy in C2C12 myotubes. The ISE and butanol (BuOH) fraction showed higher total antioxidant activity and higher total phenol content than other fractions of ISE. The ISE and BuOH fraction significantly attenuated the LPS-induced diameter of C2C12 myotubes as well as the mRNA and protein expression levels of the muscle-specific E3 ubiquitin ligases. The mRNA expression of forkhead box O type 3α was stimulated by LPS, which was suppressed by the BuOH fraction but not ISE. Furthermore, ISE and the BuOH fraction significantly reduced LPS-stimulated gene expression of pro-inflammatory cytokines and inflammation-inducible enzymes, which was mediated by through the inhibition of the p38/extracellular signal-regulated kinase signaling pathway. Thus, ISE exerts a protective effect against muscle atrophy in LPS-induced C2C12 myotubes through the antioxidant activity and anti-inflammatory effects of ISE.","PeriodicalId":48760,"journal":{"name":"Applied Sciences-Basel","volume":" ","pages":""},"PeriodicalIF":2.5000,"publicationDate":"2023-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"The Anti-Muscle Atrophy Effects of Ishige sinicola in LPS-Induced C2C12 Myotubes through Its Antioxidant and Anti-Inflammatory Actions\",\"authors\":\"Mi-Bo Kim, Hyeju Lee, Chaehyeon Lee, Yuqing Tan, Sang Gil Lee\",\"doi\":\"10.3390/app131810115\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Inflammation and oxidative stress are known to be major factors in muscle atrophy. The objective of this study was to evaluate whether the antioxidant activity of Ishige sinicola ethanol extract (ISE) and fractions from ISE could prevent lipopolysaccharide (LPS)-induced muscle atrophy in C2C12 myotubes. IS was extracted with ethanol and fractionated with five organic solvents. Then, ISE and five fractions from ISE were used to evaluate the total antioxidant activity and the protective effect of LPS-induced muscle atrophy in C2C12 myotubes. The ISE and butanol (BuOH) fraction showed higher total antioxidant activity and higher total phenol content than other fractions of ISE. The ISE and BuOH fraction significantly attenuated the LPS-induced diameter of C2C12 myotubes as well as the mRNA and protein expression levels of the muscle-specific E3 ubiquitin ligases. The mRNA expression of forkhead box O type 3α was stimulated by LPS, which was suppressed by the BuOH fraction but not ISE. Furthermore, ISE and the BuOH fraction significantly reduced LPS-stimulated gene expression of pro-inflammatory cytokines and inflammation-inducible enzymes, which was mediated by through the inhibition of the p38/extracellular signal-regulated kinase signaling pathway. Thus, ISE exerts a protective effect against muscle atrophy in LPS-induced C2C12 myotubes through the antioxidant activity and anti-inflammatory effects of ISE.\",\"PeriodicalId\":48760,\"journal\":{\"name\":\"Applied Sciences-Basel\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":2.5000,\"publicationDate\":\"2023-09-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Applied Sciences-Basel\",\"FirstCategoryId\":\"103\",\"ListUrlMain\":\"https://doi.org/10.3390/app131810115\",\"RegionNum\":4,\"RegionCategory\":\"综合性期刊\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Applied Sciences-Basel","FirstCategoryId":"103","ListUrlMain":"https://doi.org/10.3390/app131810115","RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 1

摘要

众所周知,炎症和氧化应激是肌肉萎缩的主要因素。本研究的目的是评价石斛乙醇提取物(ISE)及其提取物的抗氧化活性是否能预防脂多糖(LPS)诱导的C2C12肌管肌萎缩。用乙醇提取IS,用5种有机溶剂进行分馏。然后用ISE和ISE的5个组分评价其总抗氧化活性和对lps诱导的C2C12肌管肌萎缩的保护作用。ISE和丁醇(BuOH)部位的总抗氧化活性和总酚含量高于ISE的其他部位。ISE和BuOH组分显著降低lps诱导的C2C12肌管直径以及肌肉特异性E3泛素连接酶的mRNA和蛋白表达水平。LPS刺激叉头盒O型3α mRNA表达,BuOH组分抑制mRNA表达,ISE不抑制mRNA表达。此外,ISE和BuOH组分显著降低了lps刺激的促炎细胞因子和炎症诱导酶的基因表达,这是通过抑制p38/细胞外信号调节的激酶信号通路介导的。由此可见,ISE通过其抗氧化和抗炎作用,对lps诱导的C2C12肌管肌萎缩具有保护作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
The Anti-Muscle Atrophy Effects of Ishige sinicola in LPS-Induced C2C12 Myotubes through Its Antioxidant and Anti-Inflammatory Actions
Inflammation and oxidative stress are known to be major factors in muscle atrophy. The objective of this study was to evaluate whether the antioxidant activity of Ishige sinicola ethanol extract (ISE) and fractions from ISE could prevent lipopolysaccharide (LPS)-induced muscle atrophy in C2C12 myotubes. IS was extracted with ethanol and fractionated with five organic solvents. Then, ISE and five fractions from ISE were used to evaluate the total antioxidant activity and the protective effect of LPS-induced muscle atrophy in C2C12 myotubes. The ISE and butanol (BuOH) fraction showed higher total antioxidant activity and higher total phenol content than other fractions of ISE. The ISE and BuOH fraction significantly attenuated the LPS-induced diameter of C2C12 myotubes as well as the mRNA and protein expression levels of the muscle-specific E3 ubiquitin ligases. The mRNA expression of forkhead box O type 3α was stimulated by LPS, which was suppressed by the BuOH fraction but not ISE. Furthermore, ISE and the BuOH fraction significantly reduced LPS-stimulated gene expression of pro-inflammatory cytokines and inflammation-inducible enzymes, which was mediated by through the inhibition of the p38/extracellular signal-regulated kinase signaling pathway. Thus, ISE exerts a protective effect against muscle atrophy in LPS-induced C2C12 myotubes through the antioxidant activity and anti-inflammatory effects of ISE.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Applied Sciences-Basel
Applied Sciences-Basel CHEMISTRY, MULTIDISCIPLINARYMATERIALS SCIE-MATERIALS SCIENCE, MULTIDISCIPLINARY
CiteScore
5.30
自引率
11.10%
发文量
10882
期刊介绍: Applied Sciences (ISSN 2076-3417) provides an advanced forum on all aspects of applied natural sciences. It publishes reviews, research papers and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
期刊最新文献
Application of Digital Holographic Imaging to Monitor Real-Time Cardiomyocyte Hypertrophy Dynamics in Response to Norepinephrine Stimulation. Study on Shear Resistance and Structural Performance of Corrugated Steel–Concrete Composite Deck Clustering Analysis of Wind Turbine Alarm Sequences Based on Domain Knowledge-Fused Word2vec Unraveling Functional Dysphagia: A Game-Changing Automated Machine-Learning Diagnostic Approach Spatial Overlay Analysis of Geochemical Singularity Index α-Value of Porphyry Cu Deposit in Gangdese Metallogenic Belt, Tibet, Western China
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1