石重菌通过抗氧化和抗炎作用对脂多糖诱导的C2C12肌管的抗萎缩作用

IF 2.5 4区 综合性期刊 Q2 CHEMISTRY, MULTIDISCIPLINARY Applied Sciences-Basel Pub Date : 2023-09-08 DOI:10.3390/app131810115
Mi-Bo Kim, Hyeju Lee, Chaehyeon Lee, Yuqing Tan, Sang Gil Lee
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引用次数: 1

摘要

众所周知,炎症和氧化应激是肌肉萎缩的主要因素。本研究的目的是评价石斛乙醇提取物(ISE)及其提取物的抗氧化活性是否能预防脂多糖(LPS)诱导的C2C12肌管肌萎缩。用乙醇提取IS,用5种有机溶剂进行分馏。然后用ISE和ISE的5个组分评价其总抗氧化活性和对lps诱导的C2C12肌管肌萎缩的保护作用。ISE和丁醇(BuOH)部位的总抗氧化活性和总酚含量高于ISE的其他部位。ISE和BuOH组分显著降低lps诱导的C2C12肌管直径以及肌肉特异性E3泛素连接酶的mRNA和蛋白表达水平。LPS刺激叉头盒O型3α mRNA表达,BuOH组分抑制mRNA表达,ISE不抑制mRNA表达。此外,ISE和BuOH组分显著降低了lps刺激的促炎细胞因子和炎症诱导酶的基因表达,这是通过抑制p38/细胞外信号调节的激酶信号通路介导的。由此可见,ISE通过其抗氧化和抗炎作用,对lps诱导的C2C12肌管肌萎缩具有保护作用。
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The Anti-Muscle Atrophy Effects of Ishige sinicola in LPS-Induced C2C12 Myotubes through Its Antioxidant and Anti-Inflammatory Actions
Inflammation and oxidative stress are known to be major factors in muscle atrophy. The objective of this study was to evaluate whether the antioxidant activity of Ishige sinicola ethanol extract (ISE) and fractions from ISE could prevent lipopolysaccharide (LPS)-induced muscle atrophy in C2C12 myotubes. IS was extracted with ethanol and fractionated with five organic solvents. Then, ISE and five fractions from ISE were used to evaluate the total antioxidant activity and the protective effect of LPS-induced muscle atrophy in C2C12 myotubes. The ISE and butanol (BuOH) fraction showed higher total antioxidant activity and higher total phenol content than other fractions of ISE. The ISE and BuOH fraction significantly attenuated the LPS-induced diameter of C2C12 myotubes as well as the mRNA and protein expression levels of the muscle-specific E3 ubiquitin ligases. The mRNA expression of forkhead box O type 3α was stimulated by LPS, which was suppressed by the BuOH fraction but not ISE. Furthermore, ISE and the BuOH fraction significantly reduced LPS-stimulated gene expression of pro-inflammatory cytokines and inflammation-inducible enzymes, which was mediated by through the inhibition of the p38/extracellular signal-regulated kinase signaling pathway. Thus, ISE exerts a protective effect against muscle atrophy in LPS-induced C2C12 myotubes through the antioxidant activity and anti-inflammatory effects of ISE.
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来源期刊
Applied Sciences-Basel
Applied Sciences-Basel CHEMISTRY, MULTIDISCIPLINARYMATERIALS SCIE-MATERIALS SCIENCE, MULTIDISCIPLINARY
CiteScore
5.30
自引率
11.10%
发文量
10882
期刊介绍: Applied Sciences (ISSN 2076-3417) provides an advanced forum on all aspects of applied natural sciences. It publishes reviews, research papers and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
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