D. Real, C. Revell, Y. Han, Chun Xing Li, M. Castello, C. Bailey
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The plants are being evaluated for flowering behaviour, seed and herbage production across two environmentally diverse sites at Carnarvon and Kununurra in northern Western Australia. Key results. During 2018/19 and 2019/20, 2260 crosses between 45 different combinations of diploid females by tetraploid males and their reciprocals have created over 3000 putative triploid hybrid plants. This is the first time that triploids have successfully been created in Australia and 10 new parental combinations were created as a world-first. Conclusions. A cohort of sterile triploid plants has been identified and selections will be made based on their edible biomass productivity, insect tolerance, capacity for regrowth after cutting, nutritive value and plant chemistry (including anti-methanogenic responses). A sterile leucaena variety will have application not only in Australian grazing systems but also in other tropical regions of the world. Implications. 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We investigated the feasibility of developing a forage variety of triploid leucaena through interspecific hybridisation that is sterile (without seeds) as a strategy to mitigate its weed risk. Methods. A conventional breeding strategy was used to create triploid hybrids from hand-crosses between tetraploid and diploid species of leucaena. Molecular markers were developed to confirm successful crosses and flow cytometry was used to confirm the ploidy level. The plants are being evaluated for flowering behaviour, seed and herbage production across two environmentally diverse sites at Carnarvon and Kununurra in northern Western Australia. Key results. During 2018/19 and 2019/20, 2260 crosses between 45 different combinations of diploid females by tetraploid males and their reciprocals have created over 3000 putative triploid hybrid plants. This is the first time that triploids have successfully been created in Australia and 10 new parental combinations were created as a world-first. 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引用次数: 1
摘要
抽象的上下文。豆科灌木/乔木leucaena (leucaena leucocephala (Lam.) de Wit.)被高度评价为牛饲料,尽管它在澳大利亚的许多地方被认为是一种环境杂草。目标研究了通过种间杂交不育(无种子)培育三倍体银合子饲草品种的可行性,以降低其杂草风险。方法。采用传统的育种策略,将四倍体和二倍体青合欢手工杂交,获得三倍体杂种。利用分子标记确认杂交成功,用流式细胞术确认倍性水平。在西澳大利亚北部卡纳文和库努努拉两个环境多样化的地点,正在评估这些植物的开花行为、种子和牧草产量。关键的结果。在2018/19和2019/20期间,在45种不同的二倍体雌性与四倍体雄性及其往复组合之间进行了2260次杂交,产生了3000多个假定的三倍体杂交植株。这是澳大利亚首次成功培育出三倍体,世界上首次培育出10个新的亲本组合。结论。已鉴定出不育三倍体植物,并将根据其可食性生物量生产力、耐虫性、刈割后再生能力、营养价值和植物化学(包括抗产甲烷反应)进行选择。不育的银合欢品种将不仅在澳大利亚的放牧系统中得到应用,而且在世界其他热带地区也将得到应用。的影响。无菌品种的商业化需要一种具有成本效益的白芨大规模无性繁殖技术。
Successful creation of seedless (sterile) leucaena germplasm developed from interspecific hybridisation for use as forage
ABSTRACT Context. The legume shrub/tree leucaena (Leucaena leucocephala (Lam.) de Wit.) is highly regarded as a cattle fodder, although it is considered an environmental weed in many parts of Australia. Aims. We investigated the feasibility of developing a forage variety of triploid leucaena through interspecific hybridisation that is sterile (without seeds) as a strategy to mitigate its weed risk. Methods. A conventional breeding strategy was used to create triploid hybrids from hand-crosses between tetraploid and diploid species of leucaena. Molecular markers were developed to confirm successful crosses and flow cytometry was used to confirm the ploidy level. The plants are being evaluated for flowering behaviour, seed and herbage production across two environmentally diverse sites at Carnarvon and Kununurra in northern Western Australia. Key results. During 2018/19 and 2019/20, 2260 crosses between 45 different combinations of diploid females by tetraploid males and their reciprocals have created over 3000 putative triploid hybrid plants. This is the first time that triploids have successfully been created in Australia and 10 new parental combinations were created as a world-first. Conclusions. A cohort of sterile triploid plants has been identified and selections will be made based on their edible biomass productivity, insect tolerance, capacity for regrowth after cutting, nutritive value and plant chemistry (including anti-methanogenic responses). A sterile leucaena variety will have application not only in Australian grazing systems but also in other tropical regions of the world. Implications. A cost-effective technology for mass vegetative propagation of leucaena will be required for commercialisation of a sterile variety.
期刊介绍:
Crop and Pasture Science (formerly known as Australian Journal of Agricultural Research) is an international journal publishing outcomes of strategic research in crop and pasture sciences and the sustainability of farming systems. The primary focus is broad-scale cereals, grain legumes, oilseeds and pastures. Articles are encouraged that advance understanding in plant-based agricultural systems through the use of well-defined and original aims designed to test a hypothesis, innovative and rigorous experimental design, and strong interpretation. The journal embraces experimental approaches from molecular level to whole systems, and the research must present novel findings and progress the science of agriculture.
Crop and Pasture Science is read by agricultural scientists and plant biologists, industry, administrators, policy-makers, and others with an interest in the challenges and opportunities facing world agricultural production.
Crop and Pasture Science is published with the endorsement of the Commonwealth Scientific and Industrial Research Organisation (CSIRO) and the Australian Academy of Science.