The Effects of Using Evogen Biogas Additive on the Microbiome and Performance of Full-Scale Biogas Plant

Biogas production from organic waste is a promising renewable energy source, but achieving optimal production and digester stability can be challenging. This study investigated the impact of the Evogen microbial additive on biogas production and digester status in two biogas plants (BG01 and BG02). Microbial abundance and physicochemical parameters were analyzed to assess the effects. The results show distinct microbial community shifts in Evogen-treated digesters, with increased abundance of methanogenic archaea and hydrolytic bacteria, indicating improved anaerobic digestion. Evogen supplementation positively influenced digester performance, as evidenced by higher alkalinity buffer capacity (FOS/TAC ratios), indicating enhanced acidification and methanogenesis, along with reductions in total solids and volatile solids, demonstrating improved organic matter degradation. Evogen-treated digesters exhibited significantly higher biogas production and improved process stability, as indicated by volatile fatty acids (VFAs) profiling. The dominance of Firmicutes, Synergistetes, Proteolytic Bacteroidetes and Actinobacteria highlighted their roles in substrate degradation and VFA production. The findings contribute to optimizing biogas production systems and understanding complex microbial interactions within anaerobic digesters. The addition of Evogen influenced microbial community composition and dynamics, potentially altering substrate utilization, metabolic interactions and overall community structure.