{"title":"结缕草ZjHSP18.9基因的克隆与表达分析","authors":"Zhi-yong Huang, Xin-bo Sun, Yao Zou","doi":"10.1111/grs.12359","DOIUrl":null,"url":null,"abstract":"<p>The <i>ZjHSP18.9</i> gene was isolated from <i>Zoysia</i>grass by homologous cloning based on publicly available transcriptome database. Sequence alignment analysis revealed that ZjHSP18.9 protein contains a classic Hsp23/ACD domain. Phylogenetic analysis and subcellular localization prediction suggested that <i>ZjHSP18.9</i> belongs to cytosolic class VI subfamily. The expression profiles of <i>ZjHSP18.9</i> in leaf and root after abiotic stress or Abscisic Acid (ABA) treatment was investigated using quantitative real-time polymerase chain reaction. The results showed that the expression level of <i>ZjHSP18.9</i> in leaf was higher than that in root. The expression of <i>ZjHSP18.9</i> was strongly induced by high temperature in leaf; the <i>ZjHSP18.9</i> expression level was up-regulated in both leaf and root by the treatment of low temperature, salt and heavy metal, while down-regulated under the treatment of drought and ABA. The gene expression profiles indicated that <i>ZjHSP18.9</i> may play an important role in <i>Zoysia</i>grass response to environmental stress including extreme temperature, salinity, water deficiency and heavy metal through ABA dependent and/or independent pathway.</p>","PeriodicalId":56078,"journal":{"name":"Grassland Science","volume":"68 3","pages":"233-239"},"PeriodicalIF":1.1000,"publicationDate":"2022-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Cloning and expression analysis of ZjHSP18.9 from Zoysia japonica\",\"authors\":\"Zhi-yong Huang, Xin-bo Sun, Yao Zou\",\"doi\":\"10.1111/grs.12359\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>The <i>ZjHSP18.9</i> gene was isolated from <i>Zoysia</i>grass by homologous cloning based on publicly available transcriptome database. Sequence alignment analysis revealed that ZjHSP18.9 protein contains a classic Hsp23/ACD domain. Phylogenetic analysis and subcellular localization prediction suggested that <i>ZjHSP18.9</i> belongs to cytosolic class VI subfamily. The expression profiles of <i>ZjHSP18.9</i> in leaf and root after abiotic stress or Abscisic Acid (ABA) treatment was investigated using quantitative real-time polymerase chain reaction. The results showed that the expression level of <i>ZjHSP18.9</i> in leaf was higher than that in root. The expression of <i>ZjHSP18.9</i> was strongly induced by high temperature in leaf; the <i>ZjHSP18.9</i> expression level was up-regulated in both leaf and root by the treatment of low temperature, salt and heavy metal, while down-regulated under the treatment of drought and ABA. The gene expression profiles indicated that <i>ZjHSP18.9</i> may play an important role in <i>Zoysia</i>grass response to environmental stress including extreme temperature, salinity, water deficiency and heavy metal through ABA dependent and/or independent pathway.</p>\",\"PeriodicalId\":56078,\"journal\":{\"name\":\"Grassland Science\",\"volume\":\"68 3\",\"pages\":\"233-239\"},\"PeriodicalIF\":1.1000,\"publicationDate\":\"2022-02-21\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Grassland Science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/grs.12359\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"AGRICULTURE, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Grassland Science","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/grs.12359","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"AGRICULTURE, MULTIDISCIPLINARY","Score":null,"Total":0}
Cloning and expression analysis of ZjHSP18.9 from Zoysia japonica
The ZjHSP18.9 gene was isolated from Zoysiagrass by homologous cloning based on publicly available transcriptome database. Sequence alignment analysis revealed that ZjHSP18.9 protein contains a classic Hsp23/ACD domain. Phylogenetic analysis and subcellular localization prediction suggested that ZjHSP18.9 belongs to cytosolic class VI subfamily. The expression profiles of ZjHSP18.9 in leaf and root after abiotic stress or Abscisic Acid (ABA) treatment was investigated using quantitative real-time polymerase chain reaction. The results showed that the expression level of ZjHSP18.9 in leaf was higher than that in root. The expression of ZjHSP18.9 was strongly induced by high temperature in leaf; the ZjHSP18.9 expression level was up-regulated in both leaf and root by the treatment of low temperature, salt and heavy metal, while down-regulated under the treatment of drought and ABA. The gene expression profiles indicated that ZjHSP18.9 may play an important role in Zoysiagrass response to environmental stress including extreme temperature, salinity, water deficiency and heavy metal through ABA dependent and/or independent pathway.
Grassland ScienceAgricultural and Biological Sciences-Agronomy and Crop Science
CiteScore
2.70
自引率
7.70%
发文量
38
审稿时长
>12 weeks
期刊介绍：
Grassland Science is the official English language journal of the Japanese Society of Grassland Science. It publishes original research papers, review articles and short reports in all aspects of grassland science, with an aim of presenting and sharing knowledge, ideas and philosophies on better management and use of grasslands, forage crops and turf plants for both agricultural and non-agricultural purposes across the world. Contributions from anyone, non-members as well as members, are welcome in any of the following fields:
grassland environment, landscape, ecology and systems analysis;
pasture and lawn establishment, management and cultivation;
grassland utilization, animal management, behavior, nutrition and production;
forage conservation, processing, storage, utilization and nutritive value;
physiology, morphology, pathology and entomology of plants;
breeding and genetics;
physicochemical property of soil, soil animals and microorganisms and plant
nutrition;
economics in grassland systems.
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