有机种植的大麻(cannabis sativa L.)植物在花序和茎组织中含有多种可培养的附生真菌和内生真菌

IF 1 4区 生物学 Q3 PLANT SCIENCES Botany Pub Date : 2023-02-18 DOI:10.1139/cjb-2022-0116
Z. Punja, C. Scott
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引用次数: 2

摘要

研究了2019-2021年不列颠哥伦比亚省有机种植的大麻开花植物花序上作为附生植物和茎组织中作为内生植物的真菌种类的多样性。在生产期间的不同时间获得新鲜和干燥的花序样品,而在收获时获得茎。通过将含有马铃薯葡萄糖琼脂+硫酸链霉素的培养皿置于生长环境中1小时,并对土壤样品进行稀释,以评估土壤真菌多样性,从而评估空气中的真菌种类。从rDNA的ITS1-5.8S- ITS2区域的pcr衍生序列中鉴定菌落。从花序中分离得到26属29种,从茎组织分离得到11属17种。在花序上发现的大约96%的物种存在于空气中,45%存在于有机土壤中。真菌包括植物病原体、腐生菌和机会性人类病原体。在有机设施的空气和土壤中发现的大部分物种存在于大麻花序上,在那里它们可能增加总菌落形成单位并对产品质量产生负面影响。有些物种可能会导致人类过敏或继发感染。内生菌在有机种植的大麻植物中的潜在益处仍未被探索。
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Organically grown cannabis (Cannabis sativa L.) plants contain a diverse range of culturable epiphytic and endophytic fungi in inflorescences and stem tissues
We investigated the diversity of fungal species present on inflorescences as epiphytes and in stem tissues as endophytes in flowering plants of cannabis grown organically in British Columbia during 2019-2021. Fresh and dried inflorescence samples were obtained at various times during production while stems were obtained at harvest. Fungal species in the air were assessed by exposing Petri dishes containing potato dextrose agar + streptomycin sulphate for 1 hr in the growing environment while soil samples were dilution-plated to assess soil fungal diversity. Colonies were identified from PCR-derived sequences of the ITS1-5.8S- ITS2 region of rDNA. Twenty-nine species in 26 genera were recovered from inflorescences and 17 species in 11 genera originated from stem tissues. Approximately 96 % of species found on inflorescences were present in air and 45 % were present in organic soil. The fungi comprised plant pathogens, saprophytes, and opportunistic human pathogens. A large proportion of the species found in air and soil in organic facilities are present on cannabis inflorescences, where they may increase total colony- forming units and negatively affect product quality. Some species could contribute to allergies or secondary infections in humans. The potential benefits of endophytes within organically grown cannabis plants remain unexplored.
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来源期刊
Botany
Botany 生物-植物科学
CiteScore
2.20
自引率
9.10%
发文量
48
期刊介绍: Botany features comprehensive research articles and notes in all segments of plant sciences, including cell and molecular biology, ecology, mycology and plant-microbe interactions, phycology, physiology and biochemistry, structure and development, genetics, systematics, and phytogeography. It also publishes methods, commentary, and review articles on topics of current interest, contributed by internationally recognized scientists.
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