健康和骨关节炎马滑液中胰岛素样生长因子1(IGF-1)的浓度及其与促炎细胞因子IL-6和TNF的相关性

IF 0.7 4区 农林科学 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE Revista Mexicana de Ciencias Pecuarias Pub Date : 2022-12-27 DOI:10.22319/rmcp.v14i1.6241
Fernando García-Lacy F., Sara Teresa Méndez-Cruz, Horacio Reyes-Vivas, Víctor Manuel Dávila-Borja, José Alejandro Barrera-Morales, Gabriel Gutiérrez-Ospina, M. Gómez-Chavarín, F. Trigo-Tavera
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引用次数: 0

摘要

胰岛素样生长因子I(IGF-1)是已知的用于马软骨修复的最重要的生长因子。它促进软骨细胞的有丝分裂、II型胶原的表达和细胞外基质的产生。骨关节炎(OA)是最常见的肌肉骨骼疾病,会导致运动马跛脚和表现不佳。共对11匹跛脚马进行了临床和放射学评估,通过阳性屈曲试验、低4点神经阻滞和关节内阻滞,所有跛脚马都被证实患有前掌指跛脚。ELISA法测定总蛋白、IGF-1、IL-6和TNFa,显示临床状况、放射学变化和炎症程度之间的变化和不同的相关性。所有患有关节相关疼痛并因此致残的马的总蛋白(P<0.0001)和IGF-1浓度(P<0.05)均显著增加。对照组和跛马的IL-6和TNFa浓度存在显著差异(分别为P<0.01和P<0.001)。放射学变化较少的马在滑液中表现出最高的IGF-1表达,而患有更多慢性OA的马与对照关节相比,IGF-1表达水平非常相似。在所有跛脚关节中,通过蛋白质印迹鉴定出与炎症相关的较轻的IGF-1亚型(~7.5kDa),它是成熟肽的分子量,并且所有对照关节表达较重的亚型(~12kDa。这一发现可能会导致对关节炎症过程中不表达的同种型进行测序和靶向的新研究,并更好地了解其在马关节中的作用。
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Concentraciones del factor de crecimiento similar a la insulina 1 (IGF-1) en el líquido sinovial de caballos sanos y osteoartríticos, y su correlación con las citoquinas proinflamatorias IL-6 y TNF
Insulin-like growth factor I (IGF-1) is the most important known growth factor for cartilage repair in horses. It promotes mitosis of chondrocytes, collagen II expression, and extra cellular matrix production. Osteoarthritis (OA) is the most common musculoskeletal condition that causes lameness and poor performance in sport horses. A total of 11 lame horses were clinically and radiographically evaluated, and all were confirmed to suffer a front metacarpophalangeal lameness by a positive flexion test, a low-4-point nerve block and an intraarticular block. Total protein, IGF-1, IL-6 and TNFa were determined by ELISA, demonstrating changes and different correlations between clinical condition, radiographic changes and degree of inflammation. All horses with joint associated pain and therefore associated lameness, demonstrated a significant increase of total protein (P<0.0001) and IGF-1 concentration (P<0.05). Concentrations of IL-6 and TNFa between controls and lame horses demonstrated significant differences (P<0.01 and P<0.001 respectively). Horses with less radiographic changes, demonstrated the highest IGF-1 expression in synovial fluid, and horses with more chronic OA conditions had very similar IGF-1 expression levels than control joints. In all lame joints, it was identified by Western blot a lighter isoform of IGF-1 (~7.5 kDa) which was inflammation related and it is the molecular weight of the mature peptide, and all control joints expressed a heavier isoform (~12 kDa). This finding could lead to new research for sequencing and targeting the isoform which is not expressed during an inflammatory process within a joint, and to have a better understanding of its role in the horse’s joint.
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来源期刊
Revista Mexicana de Ciencias Pecuarias
Revista Mexicana de Ciencias Pecuarias AGRICULTURE, DAIRY & ANIMAL SCIENCE-
CiteScore
1.20
自引率
0.00%
发文量
93
审稿时长
>12 weeks
期刊介绍: The MEXICAN MAGAZINE OF SCIENCES PECUARIAS is an organ of scientific and technical diffusion of the livestock sector. Its periodicity is quarterly and arbitrated by pairs in the double-blind mode. Its objective is to make known the results of the research carried out by any scientific institution, in Mexico and in any part of the world, related to the livestock sciences, particularly those that refer to the different disciplines of Veterinary Medicine and Animal Science. The Journal is bilingual, publishes the complete articles in Spanish or English and is included in various indexing services and international dissemination platforms, such as the Index of Mexican Journals of Scientific and Technological Research of the National Council of Science and Technology (CONACYT); In the EBSCO Host database; In the Network of Scientific Journals of Latin America and the Caribbean, Spain and Portugal (RedALyC); In the Ibero-American Network of Scientific Journals of Free Access Veterinary Medicine. Indexed in the ISI Journal Citation Report Science Edition; And Elsevier''s SCOPUS and EMBASE indices.
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