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{"title":"巴基斯坦Jhang自然感染驯养狗和流浪狗犬肝人畜共患病分子诊断和系统发育分析的首次报告","authors":"M. Abbas","doi":"10.17957/ijab/15.1803","DOIUrl":null,"url":null,"abstract":"Canine diseases, particularly caused by tick-borne hepatozoons are responsible for high morbidity and mortality and are the reason for attracting significant focus. The current study was aimed to survey the occurrence of H. canis in domesticated and stray dogs of Jhang city (Punjab) with its molecular identification. For this, blood samples from 300 dogs (n=200 domesticated; n=100 stray) were collected and assayed through PCR for the detection of H. canis supported by sequencing analysis. The results demonstrated, 15.66% (47/300) of samples positive for H. canis. A significantly (P<0.05) higher occurrence of H. canis was observed in stray dogs (27%) than domesticated dogs (10%). Evaluation of the various risk factors showed that the age, sex, breed, dog category (domestic or stray), body coat, environmental settings (rural or urban) and ectoparasitic infestation were significantly (P < 0.05) associated with the occurrence of infection. The phylogenetic analysis of the PCR confirmed specimens revealed a very close homology of the detected strains with the ones diagnosed earlier in China and Malta. It was concluded that present moderate prevalence of H. canis among the dog populations in the area of Jhang may rise with stray dogs being most vulnerable hosts and potential source of vectors spread. © 2021 Friends Science Publishers","PeriodicalId":13769,"journal":{"name":"International Journal of Agriculture and Biology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"First Report on Molecular Diagnosis and Phylogenetic Analysis of Hepatozoon canis in Naturally Infected Domesticated and Stray Dogs from Jhang, Pakistan\",\"authors\":\"M. Abbas\",\"doi\":\"10.17957/ijab/15.1803\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Canine diseases, particularly caused by tick-borne hepatozoons are responsible for high morbidity and mortality and are the reason for attracting significant focus. The current study was aimed to survey the occurrence of H. canis in domesticated and stray dogs of Jhang city (Punjab) with its molecular identification. For this, blood samples from 300 dogs (n=200 domesticated; n=100 stray) were collected and assayed through PCR for the detection of H. canis supported by sequencing analysis. The results demonstrated, 15.66% (47/300) of samples positive for H. canis. A significantly (P<0.05) higher occurrence of H. canis was observed in stray dogs (27%) than domesticated dogs (10%). Evaluation of the various risk factors showed that the age, sex, breed, dog category (domestic or stray), body coat, environmental settings (rural or urban) and ectoparasitic infestation were significantly (P < 0.05) associated with the occurrence of infection. The phylogenetic analysis of the PCR confirmed specimens revealed a very close homology of the detected strains with the ones diagnosed earlier in China and Malta. It was concluded that present moderate prevalence of H. canis among the dog populations in the area of Jhang may rise with stray dogs being most vulnerable hosts and potential source of vectors spread. © 2021 Friends Science Publishers\",\"PeriodicalId\":13769,\"journal\":{\"name\":\"International Journal of Agriculture and Biology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2021-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Agriculture and Biology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.17957/ijab/15.1803\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"Agricultural and Biological Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Agriculture and Biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.17957/ijab/15.1803","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
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First Report on Molecular Diagnosis and Phylogenetic Analysis of Hepatozoon canis in Naturally Infected Domesticated and Stray Dogs from Jhang, Pakistan
Canine diseases, particularly caused by tick-borne hepatozoons are responsible for high morbidity and mortality and are the reason for attracting significant focus. The current study was aimed to survey the occurrence of H. canis in domesticated and stray dogs of Jhang city (Punjab) with its molecular identification. For this, blood samples from 300 dogs (n=200 domesticated; n=100 stray) were collected and assayed through PCR for the detection of H. canis supported by sequencing analysis. The results demonstrated, 15.66% (47/300) of samples positive for H. canis. A significantly (P<0.05) higher occurrence of H. canis was observed in stray dogs (27%) than domesticated dogs (10%). Evaluation of the various risk factors showed that the age, sex, breed, dog category (domestic or stray), body coat, environmental settings (rural or urban) and ectoparasitic infestation were significantly (P < 0.05) associated with the occurrence of infection. The phylogenetic analysis of the PCR confirmed specimens revealed a very close homology of the detected strains with the ones diagnosed earlier in China and Malta. It was concluded that present moderate prevalence of H. canis among the dog populations in the area of Jhang may rise with stray dogs being most vulnerable hosts and potential source of vectors spread. © 2021 Friends Science Publishers