样本处理延迟对新生儿脐带血脑损伤血清生物标志物价值的影响

Q3 Medicine Open Biomarkers Journal Pub Date : 2019-02-15 DOI:10.2174/1875318301909010021
M. Weiss, N. Bliznyuk, Candace Rossignol, Livia Sura, Melissa Huene, N. Copenhaver, Olena Y. Glushakova, R. Hayes
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引用次数: 0

摘要

当新生儿出生时疑似脑损伤,通常从脐带血中提取血液样本,但并不总是立即处理。测试脑损伤生物标志物分析在经历延迟处理的样本上的准确性。没有脑损伤危险因素的健康新生儿提供了脐带血样本。1组血样立即离心,取血清,分别于基线、4、8小时冷冻。第2组立即处理基线样本,然后血液样本与凝块部分保持接触,直到4、8小时,然后离心。酶联免疫吸附法测定泛素c端水解酶L1 (UCH-L1)和胶质纤维酸性蛋白(GFAP)的浓度。组1在0 h时GFAP平均浓度为62±47 pg/ml (n=32),在4和8 h时平均升高3±14%,下降0.2±9%。0 h时UCH-L1平均浓度为3306±3093 pg/ml (n=37), 4 h时平均升高3±10%,8 h时平均下降0.6±11%。组2在0 h时平均GFAP浓度为104±111 pg/ml (n=9),在4和8 h时平均分别下降5±9%和7±7%。0 h时UCH-L1平均浓度为3448±2456 pg/ml (n=8), 4 h和8 h时平均浓度分别增加9±6%和6±18%。处理延迟8小时对UCH-L1或GFAP的浓度没有显著影响。
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Effect of Sample Processing Delays on the Values of Serum Based Biomarkers of Brain Injury Collected from the Umbilical Cord Blood of Neonates
When a neonate is born with suspected brain injury, blood samples are often obtained from the umbilical cord blood but are not always processed immediately. Test the accuracy of brain injury biomarker assays on samples that experienced delayed processing. Healthy neonates who did not have risk factors for brain injury provided cord blood samples. Group 1 blood samples were centrifuged immediately, and the serum was removed and frozen at baseline, 4, and 8 hours. Group 2 had a baseline sample processed immediately and then blood samples remained in contact with the clotted portion until 4, and 8 hours and then were centrifuged. Enzyme-linked immunosorbent assays determined the concentrations of Ubiquitin C-Terminal Hydrolase L1 (UCH-L1) and Glial Fibrillary Acidic Protein (GFAP). Group 1’s average concentrations of GFAP were 62±47 pg/ml at 0 hours (n=32) with a mean increase of 3±14% and a decrease of 0.2±9% at 4 and 8 hours, respectively. UCH-L1 average concentrations were 3306±3093 pg/ml at 0 hours (n=37) with a mean increase of 3±10% at 4 hours and a mean decrease of 0.6±11% at 8 hours. Group 2’s average GFAP concentrations were 104±111 pg/ml at 0 hours (n=9) with a mean decrease of 5±9% and 7±7% at 4 and 8 hours, respectively. UCH-L1 average concentrations were 3448±2456 pg/ml at 0 hour (n=8) with a mean increase of 9±6% and 6±18% at 4 and 8 hours, respectively. Delays in processing up to 8 hours did not significantly affect the concentration of UCH-L1 or GFAP.
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来源期刊
Open Biomarkers Journal
Open Biomarkers Journal Medicine-Medicine (miscellaneous)
CiteScore
0.80
自引率
0.00%
发文量
9
期刊介绍: The Open Biomarkers Journal is an Open Access online journal, which publishes original full-length, short research articles and reviews on biomarkers in clinical, medical and pharmaceutical research. The coverage includes biomarkers of disease, new biomarkers, exposure to drugs, genetic effects, and applications of biomarkers. The Open Biomarkers Journal, a peer reviewed journal, aims to provide the most complete and reliable source of information on current developments in the field. The emphasis will be on publishing quality articles rapidly and freely available to researchers worldwide.
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