牛粪中脲酶的提取及性质研究

Zusfahair Zusfahair, A. Fatoni, D. Ningsih, M. Kurniasih
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引用次数: 0

摘要

脲酶是一种生物催化剂,可将尿素水解成氨和二氧化碳。由于是进口产品,尿素的价格仍然很高。脲酶存在于谷物中。榴莲(Durio zibethinus L.)种子是尚未探索其脲酶含量的谷物之一。本研究旨在确定发芽时间对榴莲种子脲酶活性的影响及其特性,包括pH值、培养温度、酶促反应时间、EDTA和金属的添加以及储存时间对榴莲种子脲酶活力的影响。本研究的第一步是在黑暗中发芽0、3、5、7和9天。榴莲籽芽是用研钵和杵捣碎提取的。然后使用搅拌器将它们均化,并在冷状态下离心。然后使用奈斯勒法测试所获得的粗脲酶提取物的活性。使用ANOVA对所获得的数据进行统计学检验。结果表明,榴莲种子脲酶活性在发芽3天、pH7、培养温度30℃、酶促反应时间15分钟时最为适宜,酶活性为163.6U/mL。脲酶是一种金属酶,其抑制剂为Cu2+和Na+金属离子,激活剂为Ba2+金属离子。Tukey的试验分析表明,在4°C下储存时间的影响使脲酶活性稳定8天,而在室温下,脲酶活性显著降低至72.8%。
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Extraction and Characterization of Urease from Durio zibethinus L
Urease is a biocatalyst that serves to hydrolyze urea into ammonia and carbon dioxide. Since it is an imported product, the price of urea is still high. Urease can be found in grains. One of the grains that has not been explored for its urease content is durian (Durio zibethinus L.) seeds. This study aims to determine the effect of germination time on the activity of urease from durian seeds and its characteristics including the effect of pH, incubation temperature, enzymatic reaction time, addition of EDTA and metals, and storage time on the activity of urease from durian seeds. The first step of this study was seed germination which was carried out in the dark for 0, 3, 5, 7, and 9 days. Durian seed sprouts were extracted by mashing them using a mortar and pestle. They were then homogenized using a stirrer and centrifuged in a cold state. The crude urease extract obtained was then tested for its activity using the Nessler method. The acquired data was tested statistically using ANOVA. The results showed that the activity of urease from durian seeds was optimum at 3-day germination time, pH 7, incubation temperature at 30 °C, and 15-minute enzymatic reaction time with an activity of 163.6 U/mL. Urease is a metalloenzyme with its inhibitor being the Cu2+ and Na+ metal ions and its activator being Ba2+ metal ion. Tukey's test analysis showed that the effect of urease storage time at 4 °C resulted in a stable urease activity for 8 days while at room temperature it decreased its activity significantly to 72.8%.
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来源期刊
CiteScore
0.80
自引率
0.00%
发文量
15
审稿时长
24 weeks
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