Optimization of Process Parameters for High Yield Production of Exo-Inulinase from Trichoderma asperellum RSBR08 by Using Solid State Fermentation

Inulinase is an industrially applicable enzyme which is used widely in the production of ultra high fructose syrups and fructo-oligosaccharides. Production of inulinase enzyme in a cost effective manner is the major challenge faced. In the present research study, Trichoderma asperellum RSBR08 which has the capability to produce high exo-inulinase enzyme is produced by using the solid state fermentation. Process parameters for production of exo-inulinase by using solid state fermentation were optimized. Results showed high exo-inulinase production in garlic as substrate (199.2±6.3 U/gds), 45% moisture percentage (228.4±3.4), 26 o C temperature (219.1±5.8) and 5.0 pH (213.6±4.5). Effect of different metals viz. Mg 2+ ,Zn 2+ , K + ,Ca 2+ , Na + ,Mn 2+ and Hg 2+ were studied on the production of exo-inulinase enzyme in which Ca 2+ induced the enzyme production (196.3±5.6) whereas Hg 2+ has inhibited the inulinase production (37.0±3.3). Based on the above results, the solid substrate fermentation parameters optimized can be used industrially.