基于基因组分析的裂褶菌群落微卫星标记设计

S. Boiko
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引用次数: 0

摘要

DNA简单重复序列(SSRs)是群体遗传学、系统遗传学和遗传作图中最常用的遗传标记来源。鉴定出大量富集于G和C的核苷酸重复序列。记录了336个重复次数超过10次的单核苷酸基序。共鉴定出2020个核苷酸重复序列,其中双核苷酸(68.2%)和三核苷酸(29.2%)占97.4%。扩增引物对的独特SSR位点总数为1920个。本文给出了南芥基因组独特SSR位点的PCR引物序列。在为S. commune基因组合成的22个SSR标记中,扩增子在新分离的DNA样本上形成64%。
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Design of microsatellite markers for Schizophyllum commune (Agaricales, Basidiomycota) based on analysis of its genome
Simple sequence repeats of DNA (SSRs) are the most popular source of genetic markers used in population genetics, phylogenetics, and genetic mapping. A large number of nucleotide repeats enriched in G and C were identified. 336 mononucleotide motifs with more than ten repeats were recorded. 2020 nucleotide repeats were identified, of which 97.4% are di- (68.2%) and trinucleotides (29.2%). The total number of unique SSR loci, to which primers pairs were developed, was 1920. PCR primer sequences for unique SSR loci of the S. commune genome are presented. Of the twenty-two SSR markers synthesized for the S. commune genome, amplicons formed 64% on freshly isolated DNA samples.
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CiteScore
0.80
自引率
0.00%
发文量
36
审稿时长
8 weeks
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