Lilium pumilum l petal xanthophylls identification problems by the reversed-phase HPLC method with spectrophotometric and mass-spectrometric detections

With the use of the reversed-phase high-performance liquid chromatography (HPLC) with diode-array and mass spectrometric detection, the species composition of xanthophylls of dried red color Lilium pumilum Delile flowers was determined. It was established that this plant was a unique source of capsorubin derivatives. The main components of the carotenoid complex were diesters of all- trans and cis -isomers of capsorubin (about 70 mol %) formed by the saturated fatty acids from capric to stearic with the largest contribution of myristic (~ 36 mol %) and lauric (~ 31 mol %) acids. In addition to diethers in the extracts, monoesters of capsorubin and capsanthin were also found (about 30 % of the peak areas). The possibilities of varying the composition of the mobile phase - acetone and propanol-2 as components with the high eluting capacity and acetonitrile and methanol as components with the lower eluting power - for the separation of diethers and differentiation of derivatives of capsanthin and capsorubin and to prevent the imposition of peaks of two series homologues were considered. The features of ion formation during the mass spectrometric detection in the mode of chemical ionization at the atmospheric pressure (APCI) were determined. The possibility of using the parameters of capsorubin diester retention to determine the thermodynamically substantiated dead time of the column was considered. Key words : capsorubin, capsanthin, esters, Lilium pumilum Delile, RP-HPLC, APCI, separation selectivity (Russian)  DOI: http://dx.doi.org/10.15826/analitika.2019.23.4.002 V.I. Deineka, T.G. Burzhynskaya, L.A. Deineka  Belgorod State National Research University, Russian Federation, 308015, Belgorod, ul. Pobedy, 85