四种根草本植物表面真菌群落的DNA代谢编码分析

IF 4.7 4区 医学 Q1 CHEMISTRY, MEDICINAL Chinese Herbal Medicines Pub Date : 2024-01-01 DOI:10.1016/j.chmed.2023.01.003
Yujie Dao, Jingsheng Yu, Meihua Yang, Jianping Han, Chune Fan, Xiaohui Pang
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引用次数: 0

摘要

目的全世界广泛用作药物和膳食补充剂的当归、肉苁蓉、人参和板蓝根容易受到真菌和霉菌毒素的污染。本研究旨在通过 DNA 代谢编码分析其真菌群落。根据药材种类将样本分为四组,包括 ASR 组、CH 组、PG 组和 PQ 组。结果 所有 12 份样品均检出真菌污染。根瘤菌(13.04%-74.03%)、曲霉(1.76%-23.92%)和镰刀菌(0.26%-15.27%)是主要的真菌属。在物种水平上确定了 10 种重要真菌,包括 2 种潜在的致毒真菌(柠檬青霉和草青霉)和 8 种人类致病真菌(Alternaria infectoria、Candida sake、Hyphopichia burtonii、Malassezia globosa、M. restricta、Rhizopus arrhizus、Rhodotorula mucilaginosa 和 Ochroconis tshawytschae)。ASR 组和 CH 组的真菌群落与其他组有显著差异,而 PG 组和 PQ 组的真菌群落则相对相似。这项研究为防止根茎类药材受到真菌和霉菌毒素污染提供了重要参考。
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DNA metabarcoding analysis of fungal community on surface of four root herbs

Objective

Angelicae Sinensis Radix (ASR, Danggui in Chinese), Cistanches Herba (CH, Roucongrong in Chinese), Ginseng Radix et Rhizoma (PG, Renshen in Chinese), and Panacis Quinquefolii Radix (PQ, Xiyangshen in Chinese), widely used as medicine and dietary supplement around the world, are susceptible to fungal and mycotoxin contamination. In this study, we aim to analyze their fungal community by DNA metabarcoding.

Methods

A total of 12 root samples were collected from three main production areas in China. The samples were divided into four groups based on herb species, including ASR, CH, PG, and PQ groups. The fungal community on the surface of four root groups was investigated through DNA metabarcoding via targeting the internal transcribed spacer 2 region (ITS2).

Results

All the 12 samples were detected with fungal contamination. Rhizopus (13.04%−74.03%), Aspergillus (1.76%−23.92%), and Fusarium (0.26%−15.27%) were the predominant genera. Ten important fungi were identified at the species level, including two potential toxigenic fungi (Penicillium citrinum and P. oxalicum) and eight human pathogenic fungi (Alternaria infectoria, Candida sake, Hyphopichia burtonii, Malassezia globosa, M. restricta, Rhizopus arrhizus, Rhodotorula mucilaginosa, and Ochroconis tshawytschae). Fungal community in ASR and CH groups was significantly different from other groups, while fungal community in PG and PQ groups was relatively similar.

Conclusion

DNA metabarcoding revealed the fungal community in four important root herbs. This study provided an important reference for preventing root herbs against fungal and mycotoxin contamination.

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来源期刊
Chinese Herbal Medicines
Chinese Herbal Medicines CHEMISTRY, MEDICINAL-
CiteScore
4.40
自引率
5.30%
发文量
629
审稿时长
10 weeks
期刊介绍: Chinese Herbal Medicines is intended to disseminate the latest developments and research progress in traditional and herbal medical sciences to researchers, practitioners, academics and administrators worldwide in the field of traditional and herbal medicines. The journal's international coverage ensures that research and progress from all regions of the world are widely included. CHM is a core journal of Chinese science and technology. The journal entered into the ESCI database in 2017, and then was included in PMC, Scopus and other important international search systems. In 2019, CHM was successfully selected for the “China Science and Technology Journal Excellence Action Plan” project, which has markedly improved its international influence and industry popularity. CHM obtained the first impact factor of 3.8 in Journal Citation Reports (JCR) in 2023.
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