{"title":"Cocos nucifera L.乙醇提取物Coir和Shell U Groh的植物化学、GC-MS分析及抗菌活性研究","authors":"M. Misrahanum, nur alfiyani, H. Helwati, S. Sadli","doi":"10.22146/mot.75750","DOIUrl":null,"url":null,"abstract":"U Groh is a coconut (Cocos nucifera L.) in which the meat has not been formed yet, and its shell is consumed as a salad by the Acehnese people. This study aims to determine the phytochemicals, GC-MS analysis, and activity of coir and shell U Groh against S. aureus and E. coli bacteria. Chemical compounds were analyzed using reagents and GC-MS. Antibacterial activity was tested using well diffusion with concentrations of 5, 10, 20, and 40%. Coir and shells contain flavonoids, tannins, and saponins. GC-MS analysis shows the coir contains 20 compounds, among others Alpha. - Bisabololoxide B-Acetate, Hexadecanoic acid, Hexadecanoic acid methyl ester, 9 octadecenoic acid methyl ester, Stigmasterol, and gamma. -Sitosterol, whereas 16 compounds for the shell Hexadecanoic acid, Hexadecanoic acid methyl ester, and Stigmast-5-en-3-ol. The highest activity of coir and shell U Groh against E. coli and S. aureus was a concentration of 40%. The antibacterial activity of U Groh coir and shell extract was more effective in inhibiting S. aureus than E. coli. Coir ethanol extract and its shell formed inhibition zones with diameters of 13.7 mm and 13.4 mm, respectively, against S. aureus, and the diameters against E. coli were 11.3 mm and 11.03 mm, respectively. The extract ethanol of coir and shell U Groh has good potential as an antibacterial.","PeriodicalId":32438,"journal":{"name":"Majalah Obat Tradisional","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2022-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Phytochemical, GC-MS Analysis, and Antibacterial Activity of Ethanol Extract Coir and Shell U Groh (Cocos nucifera L.)\",\"authors\":\"M. Misrahanum, nur alfiyani, H. Helwati, S. Sadli\",\"doi\":\"10.22146/mot.75750\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"U Groh is a coconut (Cocos nucifera L.) in which the meat has not been formed yet, and its shell is consumed as a salad by the Acehnese people. This study aims to determine the phytochemicals, GC-MS analysis, and activity of coir and shell U Groh against S. aureus and E. coli bacteria. Chemical compounds were analyzed using reagents and GC-MS. Antibacterial activity was tested using well diffusion with concentrations of 5, 10, 20, and 40%. Coir and shells contain flavonoids, tannins, and saponins. GC-MS analysis shows the coir contains 20 compounds, among others Alpha. - Bisabololoxide B-Acetate, Hexadecanoic acid, Hexadecanoic acid methyl ester, 9 octadecenoic acid methyl ester, Stigmasterol, and gamma. -Sitosterol, whereas 16 compounds for the shell Hexadecanoic acid, Hexadecanoic acid methyl ester, and Stigmast-5-en-3-ol. The highest activity of coir and shell U Groh against E. coli and S. aureus was a concentration of 40%. The antibacterial activity of U Groh coir and shell extract was more effective in inhibiting S. aureus than E. coli. Coir ethanol extract and its shell formed inhibition zones with diameters of 13.7 mm and 13.4 mm, respectively, against S. aureus, and the diameters against E. coli were 11.3 mm and 11.03 mm, respectively. The extract ethanol of coir and shell U Groh has good potential as an antibacterial.\",\"PeriodicalId\":32438,\"journal\":{\"name\":\"Majalah Obat Tradisional\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-12-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Majalah Obat Tradisional\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.22146/mot.75750\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Majalah Obat Tradisional","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.22146/mot.75750","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1
摘要
U Groh是一种椰子(Cocos nucifera L.),其肉尚未形成,其外壳被亚齐人用作沙拉食用。本研究旨在测定椰壳和贝壳U Groh的植物化学成分、GC-MS分析以及对金黄色葡萄球菌和大肠杆菌的活性。使用试剂和GC-MS分析化合物。使用浓度为5%、10%、20%和40%的阱扩散测试抗菌活性。椰子和果壳含有黄酮、单宁和皂苷。GC-MS分析表明,椰壳含有20种化合物,其中α-双酚B醋酸酯、十六酸、十六酸甲酯、9-十八碳烯酸甲酯、Stigmasterol和γ-山竹醇,而16种化合物为壳十六烷酸、十六烷酸甲酯和Stigmast-5-en-3-ol。椰壳U Groh对大肠杆菌和金黄色葡萄球菌的最高活性为40%。U Groh椰壳提取物对金黄色葡萄球菌的抑制作用大于对大肠杆菌的抑制作用。Coir乙醇提取物及其外壳形成直径分别为13.7mm和13.4mm的对金黄色葡萄球菌的抑制区,直径分别为11.3mm和11.03mm的对大肠杆菌的抑制区。椰壳提取物乙醇具有良好的抗菌潜力。
Phytochemical, GC-MS Analysis, and Antibacterial Activity of Ethanol Extract Coir and Shell U Groh (Cocos nucifera L.)
U Groh is a coconut (Cocos nucifera L.) in which the meat has not been formed yet, and its shell is consumed as a salad by the Acehnese people. This study aims to determine the phytochemicals, GC-MS analysis, and activity of coir and shell U Groh against S. aureus and E. coli bacteria. Chemical compounds were analyzed using reagents and GC-MS. Antibacterial activity was tested using well diffusion with concentrations of 5, 10, 20, and 40%. Coir and shells contain flavonoids, tannins, and saponins. GC-MS analysis shows the coir contains 20 compounds, among others Alpha. - Bisabololoxide B-Acetate, Hexadecanoic acid, Hexadecanoic acid methyl ester, 9 octadecenoic acid methyl ester, Stigmasterol, and gamma. -Sitosterol, whereas 16 compounds for the shell Hexadecanoic acid, Hexadecanoic acid methyl ester, and Stigmast-5-en-3-ol. The highest activity of coir and shell U Groh against E. coli and S. aureus was a concentration of 40%. The antibacterial activity of U Groh coir and shell extract was more effective in inhibiting S. aureus than E. coli. Coir ethanol extract and its shell formed inhibition zones with diameters of 13.7 mm and 13.4 mm, respectively, against S. aureus, and the diameters against E. coli were 11.3 mm and 11.03 mm, respectively. The extract ethanol of coir and shell U Groh has good potential as an antibacterial.