儒足苦药对四氧嘧啶诱导的糖尿病大鼠肝功能及血脂指标的影响

IF 3.3 Q2 GASTROENTEROLOGY & HEPATOLOGY Journal of Clinical and Experimental Hepatology Pub Date : 2024-05-01 DOI:10.1016/j.jceh.2021.09.012

David C. Obasi , Victor N. Ogugua

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引用次数: 0

摘要

背景Ruzu 草药苦味剂（RHB）是尼日利亚生产的一种多草药混合物，用于治疗糖尿病和其他疾病。该产品的消费者证明了它的功效，但有关 RHB 的科学信息并不多。本研究确定了 RHB 对阿脲诱导的糖尿病大鼠肝功能和血脂谱参数的影响。第 1 组为正常对照组，第 2-6 组为糖尿病组。第 2 组不进行治疗，第 3-6 组分别使用格列本脲 5 毫克/千克体重、RHB 0.14、0.29 和 0.57 毫升/千克体重进行治疗。第 7-9 组没有糖尿病，但接受了与第 4-6 组相同的治疗。成年雄性白化威斯特大鼠腹腔注射新鲜制备的阿脲，单次剂量为 120 毫克/千克体重，诱发糖尿病。结果表明，与正常组相比，糖尿病未治疗组的血糖水平显著升高（P <0.05），体重下降。肝功能和血脂检测结果显示，γ-谷氨酰转移酶（GGT）、碱性磷酸酶（ALP）、丙氨酸氨基转移酶（ALT）和天冬氨酸氨基转移酶（AST）的活性明显增加（P<0.05）；总胆红素、总胆固醇（T.CHOL）、甘油三酯（TG）、极低密度脂蛋白（VLDL）和低密度脂蛋白（LDL）水平升高；与正常组相比，糖尿病未治疗组的总蛋白、白蛋白和高密度脂蛋白（HDL）水平降低。然而，用不同剂量的 RHB 治疗糖尿病大鼠可使这些影响逆转至接近正常水平，且呈剂量依赖性。

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Effect of Ruzu Herbal Bitters on the Liver Function and Lipid Profile Parameters of Alloxan-Induced Diabetic Rats

Background

Ruzu herbal bitters (RHB) is a polyherbal mixture produced in Nigeria indicated for diabetes and other ailments. The consumers of the product testify of its efficacy, but there are not much scientific information on RHB. The study determined the effect of RHB on the liver function and lipid profile parameters of alloxan-induced diabetic rats.

Method

Fifty-four adult albino rats were divided into nine groups of six rats each. Group 1 was the normal control, while groups 2–6 were diabetic. Group 2 was not treated, while groups 3–6 were respectively treated with 5 mg/kg b.w of glibenclamide, 0.14, 0.29, and 0.57 ml/kg b.w of RHB. Groups 7–9 were not diabetic but treated as groups 4–6. Diabetes was induced by intraperitoneal injection of freshly prepared alloxan into adult male albino Wister rats with a single dose of 120 mg/kg body weight. The blood sugar level, weight, liver function, and lipid profile of the rats were tested using standard methods.

Result

The results showed a significant (P < 0.05) increase in the blood glucose level and decrease in weight in the diabetic-untreated group compared to the normal group. The liver function and lipid profile tests showed significant (P<0.05) increases in the activities of gamma-glutamyltransferase (GGT), alkaline phosphatase (ALP), alanine aminotransferase (ALT), and aspartate aminotransferase (AST); increases in the levels of total bilirubin, total cholesterol (T.CHOL), triglycerides (TG), very low-density lipoprotein (VLDL) and lowdensity lipoprotein (LDL); decreases in the levels of total protein, albumin and high-density lipoproteins (HDL), in the diabetic-untreated group compared to the normal group. However, treatment of the diabetic rats with different doses of RHB caused the reversal of these effects to near-normal levels in a dose-dependent manner.