脊髓损伤雌性小鼠的运动活性和肢体痉挛水平

Yu. R. Nesterenko, O. Rybachuk
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摘要

脊髓损伤(SCI)会导致大规模的身体、生理、心理和职业损失。因此,今天神经生理学的一个紧迫目标是研究脊髓损伤的后果。脊髓损伤最常见的类型是半切断术。对于脊髓创伤后功能恢复的全面和详细的研究,开发了各种半切方案。本研究的目的是分析脊髓损伤后小鼠同侧后肢(IH)的运动活动水平和痉挛变化。材料和方法。雌性FVB小鼠左脊髓半切面损伤模型建立在胸椎下部(T10-T11)水平。在创伤后的前1-12周,每周采用Basso- beattie - bresnahan (ВВВ)、Basso (B)量表和Ashworth量表测定IH的运动活动和痉挛程度。结果。在较晚的日期(第11周,第12周)与创伤后第一周相比,IH功能明显恢复:BBB评分为4.39±0.61分(21分),B评分为2.22±0.31分(9分)。然而,在脊髓损伤后的所有研究时间间隔内,实验动物的后肢痉挛水平持续较高,在第12周的Ashworth量表上为3.03±0.39(满分4分)。结论。通过半切脊髓损伤建模后,观察到创伤后运动活动的自发恢复(从第二周开始),其得分与早期恢复阶段相对应。另一方面,小鼠的IH痉挛水平在整个研究期间都相当高,尽管与创伤后第一周相比,该参数在第二周已经略有下降。
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Locomotor activity and spasticity level of the limb in female mice with a spinal cord injury model
Spinal cord injuries (SCI) lead to large-scale physical, physiological, psychological and professional losses. Therefore, today, one of the urgent aims of neurophysiology is the study of the consequences of spinal cord injury. The most common type of SCI is a hemisection. For a comprehensive and detailed study of the functional post-traumatic recovery of the spinal cord, various hemisection options are developed. The aim of the study was to analyse the level of locomotor activity and changes in spasticity of the mice ipsilateral hindlimb (IH) after SCI. Materials and methods. The injury of the left-side spinal cord hemisection was modelled at the level of the lower thoracic segments (T10-T11) in female FVB mice. The locomotor activity and spasticity of the IH were determined every week using the Basso-Beattie-Bresnahan (ВВВ), the Basso (B) scales and the Ashworth scale during the first 1-12 weeks of the post-traumatic period. Results. At a later date (the 11th, 12th weeks) compared with the first weeks of the post-traumatic period, there was a marked recovery of the IH function: 4.39±0.61 points (out of 21 possible) on the BBB scale and 2.22±0.31 points (out of 9 possible) on the B scale. However, at all studied time interval after SCI, a consistently high level of hindlimb spasticity in experimental animals was noted, on the 12th week: 3.03±0.39 (out of 4 possible) on the Ashworth scale. Conclusions. After modelling spinal cord injury by hemisection, spontaneous post-traumatic recovery of locomotor activity was observed (since the 2nd week), which had scores corresponding to the early recovery phase. On the other hand, the levels of IH spasticity in mice were quite high throughout the entire study period, although already at the 2nd week there was a slight decrease in this parameter compared to the 1st week of the post-traumatic period.
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Cell and Organ Transplantology
Cell and Organ Transplantology Medicine-Transplantation
CiteScore
0.40
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0.00%
发文量
8
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