Mina Gholami, Zahra Elyasigorji, Abdolreza Daneshvar Amoli, Parvaneh Farzaneh
{"title":"小茴香提取物对人胃癌细胞HSP90α和HER2基因表达水平的影响","authors":"Mina Gholami, Zahra Elyasigorji, Abdolreza Daneshvar Amoli, Parvaneh Farzaneh","doi":"10.1007/s13596-022-00657-z","DOIUrl":null,"url":null,"abstract":"<div><p>Gastric cancer was classified as the third most deadly cancer among all other cancer types. The HSP90 and HER2 genes play essential roles in the stability and function of high-expression proteins that cause malignancy. The aim of this research was to investigate the influence of the alcoholic <i>Alkanna bracteosa</i> extract on the expression of HSP90α and HER2 genes in AGS cell line. Therefore, the methanolic extraction was isolated from aerial parts of the plant and AGS and HuGu cell lines were analyzed using 102.4–0.05 mg ml<sup>−1</sup> dose concentrations in serial dilution; to measure the cell toxicity by MTT assay. Furthermore, real-time PCR analysis measured the expression level of HSP90α and HER2 genes using the IC50 dose concentrations. Quantification of apoptosis was analyzed by Annexin/PI kit in flow cytometry and DNA fragmentation tests. The results of MTT assay represented the IC50 dose concentration of 0.8 and 3.2 mg ml<sup>−1</sup> for AGS and HuGu respectively. The rate of HER2 gene expression was significantly decreased in AGS cells treated with 0.8 mg ml<sup>−1</sup> dose concentration compared to control. The exposure of AGS treated cells with 0.8 mg ml<sup>−1</sup> dose concentration after 24 h represented 24.3% apoptosis and 13.3% necrosis. The agarose gel represented the DNA fragmentation pattern of apoptosis. This study demonstrated the significant differences between the cell viability rate, gene expression level, and apoptosis of the <i>Alkanna bracteosa</i> extract on AGS cells. These results demonstrated the first report of which the <i>Alkanna braceteosa</i> would be an effective candidate for possible treatment of Gastric cancer.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":null,"pages":null},"PeriodicalIF":1.8000,"publicationDate":"2022-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effects of Alkanna bracteosa extract on the expression level of HSP90α and HER2 genes in human gastric cancer cell line\",\"authors\":\"Mina Gholami, Zahra Elyasigorji, Abdolreza Daneshvar Amoli, Parvaneh Farzaneh\",\"doi\":\"10.1007/s13596-022-00657-z\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Gastric cancer was classified as the third most deadly cancer among all other cancer types. The HSP90 and HER2 genes play essential roles in the stability and function of high-expression proteins that cause malignancy. The aim of this research was to investigate the influence of the alcoholic <i>Alkanna bracteosa</i> extract on the expression of HSP90α and HER2 genes in AGS cell line. Therefore, the methanolic extraction was isolated from aerial parts of the plant and AGS and HuGu cell lines were analyzed using 102.4–0.05 mg ml<sup>−1</sup> dose concentrations in serial dilution; to measure the cell toxicity by MTT assay. Furthermore, real-time PCR analysis measured the expression level of HSP90α and HER2 genes using the IC50 dose concentrations. Quantification of apoptosis was analyzed by Annexin/PI kit in flow cytometry and DNA fragmentation tests. The results of MTT assay represented the IC50 dose concentration of 0.8 and 3.2 mg ml<sup>−1</sup> for AGS and HuGu respectively. The rate of HER2 gene expression was significantly decreased in AGS cells treated with 0.8 mg ml<sup>−1</sup> dose concentration compared to control. The exposure of AGS treated cells with 0.8 mg ml<sup>−1</sup> dose concentration after 24 h represented 24.3% apoptosis and 13.3% necrosis. The agarose gel represented the DNA fragmentation pattern of apoptosis. This study demonstrated the significant differences between the cell viability rate, gene expression level, and apoptosis of the <i>Alkanna bracteosa</i> extract on AGS cells. These results demonstrated the first report of which the <i>Alkanna braceteosa</i> would be an effective candidate for possible treatment of Gastric cancer.</p></div>\",\"PeriodicalId\":7613,\"journal\":{\"name\":\"Advances in Traditional Medicine\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.8000,\"publicationDate\":\"2022-09-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Advances in Traditional Medicine\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s13596-022-00657-z\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"PHARMACOLOGY & PHARMACY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Advances in Traditional Medicine","FirstCategoryId":"1085","ListUrlMain":"https://link.springer.com/article/10.1007/s13596-022-00657-z","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PHARMACOLOGY & PHARMACY","Score":null,"Total":0}
Effects of Alkanna bracteosa extract on the expression level of HSP90α and HER2 genes in human gastric cancer cell line
Gastric cancer was classified as the third most deadly cancer among all other cancer types. The HSP90 and HER2 genes play essential roles in the stability and function of high-expression proteins that cause malignancy. The aim of this research was to investigate the influence of the alcoholic Alkanna bracteosa extract on the expression of HSP90α and HER2 genes in AGS cell line. Therefore, the methanolic extraction was isolated from aerial parts of the plant and AGS and HuGu cell lines were analyzed using 102.4–0.05 mg ml−1 dose concentrations in serial dilution; to measure the cell toxicity by MTT assay. Furthermore, real-time PCR analysis measured the expression level of HSP90α and HER2 genes using the IC50 dose concentrations. Quantification of apoptosis was analyzed by Annexin/PI kit in flow cytometry and DNA fragmentation tests. The results of MTT assay represented the IC50 dose concentration of 0.8 and 3.2 mg ml−1 for AGS and HuGu respectively. The rate of HER2 gene expression was significantly decreased in AGS cells treated with 0.8 mg ml−1 dose concentration compared to control. The exposure of AGS treated cells with 0.8 mg ml−1 dose concentration after 24 h represented 24.3% apoptosis and 13.3% necrosis. The agarose gel represented the DNA fragmentation pattern of apoptosis. This study demonstrated the significant differences between the cell viability rate, gene expression level, and apoptosis of the Alkanna bracteosa extract on AGS cells. These results demonstrated the first report of which the Alkanna braceteosa would be an effective candidate for possible treatment of Gastric cancer.
期刊介绍:
Advances in Traditional Medicine (ADTM) is an international and peer-reviewed journal and publishes a variety of articles including original researches, reviews, short communications, and case-reports. ADTM aims to bridging the gap between Traditional knowledge and medical advances. The journal focuses on publishing valid, relevant, and rigorous experimental research and clinical applications of Traditidnal Medicine as well as medical classics. At the same time, the journal is devoted to communication among basic researcher and medical clinician interested in the advancement of Traditional Medicine. Topics covered by the journal are: Medical Classics & History; Biomedical Research; Pharmacology & Toxicology of Natural Products; Acupuncture & Moxibustion; Sasang Constitutional Medicine; Diagnostics and Instrumental Development; Clinical Research. ADTM is published four times yearly. The publication date of this journal is 30th March, June, September, and December.