Wei-Hong Sun, Fei-Fei Wu, Lili Cong, Mei-Yan Jin, Xian-Guo Wang
{"title":"利用简单序列重复标记评价薇迪亚属(Vicia L.)遗传多样性和群体结构","authors":"Wei-Hong Sun, Fei-Fei Wu, Lili Cong, Mei-Yan Jin, Xian-Guo Wang","doi":"10.1111/grs.12356","DOIUrl":null,"url":null,"abstract":"<p>The genetic diversity of the <i>Vicia</i> (<i>Vicia</i> L.) genus was analyzed using simple sequence repeat (SSR) markers. A total of 201 sampled individuals from 12 <i>Vicia</i> were assayed to study the genetic diversity and polymorphisms using 12 simple sequence repeat markers; 115 alleles were identified. The average observed heterozygosity (Ho) and the average expected heterozygosity (He) were 0.4283 and 0.6941, respectively. The polymorphic information content (PIC) varied from 0.8739 (V81) to 0.9579 (V97), with a mean of 0.9033, indicating that the markers were highly informative. Moreover, cluster and principal coordinate analysis (PCoA) divided the 12 species into three main clusters. Structure analysis and PCoA of <i>Vicia narbonensis</i> and <i>Vicia tibetica</i> agreed well with the cluster analysis results, and analysis of molecular variance (AMOVA) results revealed that 89% genetic variation was observed within the population and 11% was among the population. These results clarify the genetic diversity and population structure of the <i>Vicia</i>, providing useful information for understanding genetic variability and establishing a foundation for future breeding programs and genetic improvement.</p>","PeriodicalId":56078,"journal":{"name":"Grassland Science","volume":"68 3","pages":"205-213"},"PeriodicalIF":1.1000,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":"{\"title\":\"Assessment of genetic diversity and population structure of the genus Vicia (Vicia L.) using simple sequence repeat markers\",\"authors\":\"Wei-Hong Sun, Fei-Fei Wu, Lili Cong, Mei-Yan Jin, Xian-Guo Wang\",\"doi\":\"10.1111/grs.12356\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>The genetic diversity of the <i>Vicia</i> (<i>Vicia</i> L.) genus was analyzed using simple sequence repeat (SSR) markers. A total of 201 sampled individuals from 12 <i>Vicia</i> were assayed to study the genetic diversity and polymorphisms using 12 simple sequence repeat markers; 115 alleles were identified. The average observed heterozygosity (Ho) and the average expected heterozygosity (He) were 0.4283 and 0.6941, respectively. The polymorphic information content (PIC) varied from 0.8739 (V81) to 0.9579 (V97), with a mean of 0.9033, indicating that the markers were highly informative. Moreover, cluster and principal coordinate analysis (PCoA) divided the 12 species into three main clusters. Structure analysis and PCoA of <i>Vicia narbonensis</i> and <i>Vicia tibetica</i> agreed well with the cluster analysis results, and analysis of molecular variance (AMOVA) results revealed that 89% genetic variation was observed within the population and 11% was among the population. These results clarify the genetic diversity and population structure of the <i>Vicia</i>, providing useful information for understanding genetic variability and establishing a foundation for future breeding programs and genetic improvement.</p>\",\"PeriodicalId\":56078,\"journal\":{\"name\":\"Grassland Science\",\"volume\":\"68 3\",\"pages\":\"205-213\"},\"PeriodicalIF\":1.1000,\"publicationDate\":\"2022-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Grassland Science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/grs.12356\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"AGRICULTURE, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Grassland Science","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/grs.12356","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"AGRICULTURE, MULTIDISCIPLINARY","Score":null,"Total":0}
Assessment of genetic diversity and population structure of the genus Vicia (Vicia L.) using simple sequence repeat markers
The genetic diversity of the Vicia (Vicia L.) genus was analyzed using simple sequence repeat (SSR) markers. A total of 201 sampled individuals from 12 Vicia were assayed to study the genetic diversity and polymorphisms using 12 simple sequence repeat markers; 115 alleles were identified. The average observed heterozygosity (Ho) and the average expected heterozygosity (He) were 0.4283 and 0.6941, respectively. The polymorphic information content (PIC) varied from 0.8739 (V81) to 0.9579 (V97), with a mean of 0.9033, indicating that the markers were highly informative. Moreover, cluster and principal coordinate analysis (PCoA) divided the 12 species into three main clusters. Structure analysis and PCoA of Vicia narbonensis and Vicia tibetica agreed well with the cluster analysis results, and analysis of molecular variance (AMOVA) results revealed that 89% genetic variation was observed within the population and 11% was among the population. These results clarify the genetic diversity and population structure of the Vicia, providing useful information for understanding genetic variability and establishing a foundation for future breeding programs and genetic improvement.
Grassland ScienceAgricultural and Biological Sciences-Agronomy and Crop Science
CiteScore
2.70
自引率
7.70%
发文量
38
审稿时长
>12 weeks
期刊介绍:
Grassland Science is the official English language journal of the Japanese Society of Grassland Science. It publishes original research papers, review articles and short reports in all aspects of grassland science, with an aim of presenting and sharing knowledge, ideas and philosophies on better management and use of grasslands, forage crops and turf plants for both agricultural and non-agricultural purposes across the world. Contributions from anyone, non-members as well as members, are welcome in any of the following fields:
grassland environment, landscape, ecology and systems analysis;
pasture and lawn establishment, management and cultivation;
grassland utilization, animal management, behavior, nutrition and production;
forage conservation, processing, storage, utilization and nutritive value;
physiology, morphology, pathology and entomology of plants;
breeding and genetics;
physicochemical property of soil, soil animals and microorganisms and plant
nutrition;
economics in grassland systems.