一种新的综合方法研究采后盆栽蜂蜜的变化

IF 0.7 4区 农林科学 Q4 ENTOMOLOGY Sociobiology Pub Date : 2022-11-28 DOI:10.13102/sociobiology.v69i4.8251
P. Vit, B. Chuttong, N. Zawawi, María Nieves Saldaña Díaz, Jane van der Meulen, H. F. Ahmad, F. Tomás-Barberán, Gina Meccia, K. Danmek, J. E. Moreno, D. Roubik, O. Barth, D. Lachenmeier, M. Engel
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引用次数: 2

摘要

这篇关于罐蜜研究分析方法的新综述旨在为一项综合研究中在30°C下进行的35天收获后实验提供简明参考。从专业文献、官方分析化学家协会和国际蜂蜜委员会中选择了多种方法。除了地理和季节性起源外,罐蜜I.D.还包括昆虫学和植物学鉴定,后者通过乙酰乙酸或天然蜜属植物学进行鉴定。本综合研究的方法包括:1。理化分析(Aw、颜色、水分、pH、游离酸度、内酯酸度、总酸度、羟甲基糠醛(HMF)和糖,通过高效液相色谱-HPLC),2。靶向质子核磁共振1H-NMR代谢组学(糖、乙醇、HMF、脂肪族有机酸、氨基酸和植物标志物),3。生化成分(黄酮类、多酚类),4。抗氧化活性(ABTS 2,2'-叠氮双(3-乙基苯并噻唑啉-6-磺酸自由基清除测定法、DPPH 2,2-二苯基-1-苦基肼自由基清除检测法、铁还原测定法FRAP),5。微生物计数(需氧菌平板、酵母和霉菌、芽孢杆菌和乳酸菌计数),6。通过独立培养方法进行蜂蜜微生物组分析:基于扩增子测序方法的高通量细菌和真菌,7。感官评价(气味、香气、味道、持久性)和8。蜂蜜的真实性和生物表面活性剂通过相间乳液测试。还包括了另一部分,以提供使用每种方法获得的结果的基本信息。这是解释无刺蜂巢内罐式蜂蜜加工和收获后转化产生的相互作用成分所必需的。
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A Novel Integrative Methodology for Research on Pot-honey Variations During Post-harvest
This novel review of analytical methods for pot-honey research was intended to provide concise references to a 35-day post-harvest experiments at 30 °C, in an integrated study. Diverse methods were selected from specialized literature, from the AOAC (Association of Official Analytical Chemists), and the International Honey Commission. Besides the geographical and seasonal origin, the pot-honey I.D. consists of entomological and botanical identifications, the latter performed by acetolyzed or natural melissopalynology. The methods of this integrative study included: 1. Physicochemical analysis (Aw, color, moisture, pH, free acidity, lactone acidity, total acidity, hydroxymethylfurfural (HMF), and sugars by highperformance liquid chromatography HPLC), 2. Targeted proton nuclear magnetic resonance 1H-NMR metabolomics (sugars, ethanol, HMF, aliphatic organic acids, amino acids, and botanical markers), 3. Biochemical composition (flavonoids, polyphenols), 4. Antioxidant activity (ABTS 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid-free radical scavenging assay, DPPH 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay, ferric reduction assay FRAP), 5. Microbial counts (aerobic plate, yeast and mold, Bacillus, and lactic acid bacteria count), 6. Honey microbiome profiling via independent-culture method: high-throughput bacteria and fungi based on amplicon sequencing approaches, 7. Sensory evaluation (odor, aroma, taste, persistence), and 8. Honey authenticity and biosurfactant tests by an interphase emulsion. A further section was included to provide basic information on the results obtained using each method. This was needed to explain the interacting components derived from pot-honey processing within the stingless bee nest and post-harvest transformations.
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来源期刊
Sociobiology
Sociobiology 生物-昆虫学
CiteScore
1.60
自引率
11.10%
发文量
28
审稿时长
3 months
期刊介绍: SOCIOBIOLOGY publishes high quality articles that significantly contribute to the knowledge of Entomology, with emphasis on social insects. Articles previously submitted to other journals are not accepted. SOCIOBIOLOGY publishes original research papers and invited review articles on all aspects related to the biology, evolution and systematics of social and pre-social insects (Ants, Termites, Bees and Wasps). The journal is currently expanding its scope to incorporate the publication of articles dealing with other arthropods that exhibit sociality. Articles may cover a range of subjects such as ecology, ethology, morphology, population genetics, physiology, toxicology, reproduction, sociobiology, caste differentiation as well as economic impact and pest management.
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