结合金属纳米粒子和纳米体促进神经退行性疾病的生物医学成像

IF 4.1 Q2 MATERIALS SCIENCE, MULTIDISCIPLINARY Frontiers in Nanotechnology Pub Date : 2023-09-04 DOI:10.3389/fnano.2023.1236810
A. Sanna, A. Quarta, N. Pieroni, B. Parodi, F. Palermo, I. Bukreeva, M. Fratini, L. Massimi, D. Simeone, X. Le Guével, A. Bravin, E. Quintiero, I. Viola, G. Gigli, N. Kerlero de Rosbo, L. Sancey, A. Cedola
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引用次数: 0

摘要

导读:在神经退行性疾病的研究中,跟踪整个身体内特定细胞或分子的命运的可能性将是更好地了解疾病机制的复杂演变和监测治疗效果的里程碑。目前可用的技术不允许在整个三维生物环境中以高空间分辨率可视化疾病相关细胞。方法:在这里,我们展示了一种新方法的第一个验证步骤的结果:通过结合共轭纳米体抗胶质纤维酸性蛋白(GFAP)和金属纳米粒子(即2 nm金NP)与x射线相对比断层扫描(XPCT),我们将能够获得感兴趣的细胞及其周围组织、血管和神经网络的三维可视化和识别。结果:利用金属纳米粒子的x射线衰减特性和纳米体的特异性靶向能力,我们可以赋予XPCT目前所缺乏的特异性,使其不再是纯粹的形态学成像技术,而是一种分子和靶向成像技术。在本研究中,我们合成并表征了Gold-NP/GFAP纳米体以小鼠大脑星形胶质细胞为靶点。讨论:本文提出的第一次测试结果为我们提供了该方法可行性的信息,鼓励我们进行进一步的实验,以实现建立这种新的成像技术的最终目标。
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Combining metal nanoparticles and nanobodies to boost the biomedical imaging in neurodegenerative diseases
Introduction: In the study of neurodegenerative diseases, the possibility to follow the fate of specific cells or molecules within the whole body would be a milestone to better understand the complex evolution of disease mechanisms and to monitor the effects of therapies. The techniques available today do not allow the visualization of disease-relevant cells within the whole tridimensional biological context at high spatial resolution.Methods: Here we show the results from the first validation steps of a novel approach: by combining the conjugate nanobodies anti-glial fibrillary acidic protein (GFAP) and metal-nanoparticles (i.e. 2 nm gold NP) with X-ray phase contrast tomography (XPCT) we would be able to obtain a tridimensional visualization and identification of cells of interest together with the surrounding tissue and the vascular and neuronal networks.Results: By exploiting the X-ray attenuation properties of metal nanoparticles and the specific targeting capabilities of nanobodies, we could give XPCT the specificity it presently lacks, making it no longer a pure morphological but a molecular and targeted imaging technique. In our case, we synthesized and characterized Gold-NP/GFAP nanobody to target the astrocytes of mouse brain.Discussion: The results of the first tests presented in this paper have provided us with information on the feasibility of the approach, encouraging us to carry out further experiments in order to achieve the ultimate goal of setting up this new imaging technique.
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来源期刊
Frontiers in Nanotechnology
Frontiers in Nanotechnology Engineering-Electrical and Electronic Engineering
CiteScore
7.10
自引率
0.00%
发文量
96
审稿时长
13 weeks
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