{"title":"逆流色谱法从合成混合物中分离乙酰二茂铁","authors":"Xiao‐yong Lv, Haibo You, Huiyun Qiu, Mengyi Wen, Chuncui Zheng, Shengqiang Tong","doi":"10.1080/10826076.2022.2143798","DOIUrl":null,"url":null,"abstract":"Abstract The main product of acetylation of ferrocene was successfully separated and purified with high purity from synthetic mixtures by countercurrent chromatography using a selected biphasic solvent system. According to distribution performance of main product and reactant, a two-phase solvent system was selected by thin layer chromatography, in which the partition coefficient for each component was determined by HPLC. Then a two-phase solvent system consisting of petroleum ether/ethyl acetate/methanol/water (6:4:6:4, v/v) was selected. After acetylation reaction, 20 mg of synthetic mixture was subjected to countercurrent chromatography, yielding 2.1 mg of ferrocene and 8.7 mg of acetyl ferrocene with 96.6% and 97.4% purity. Results indicated that countercurrent chromatography is a very efficient method for separation and purification of target product from synthetic mixtures. GRAPHICAL ABSTRACT","PeriodicalId":16295,"journal":{"name":"Journal of Liquid Chromatography & Related Technologies","volume":null,"pages":null},"PeriodicalIF":1.0000,"publicationDate":"2022-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Separation of acetylferrocene from synthetic mixtures by countercurrent chromatography\",\"authors\":\"Xiao‐yong Lv, Haibo You, Huiyun Qiu, Mengyi Wen, Chuncui Zheng, Shengqiang Tong\",\"doi\":\"10.1080/10826076.2022.2143798\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Abstract The main product of acetylation of ferrocene was successfully separated and purified with high purity from synthetic mixtures by countercurrent chromatography using a selected biphasic solvent system. According to distribution performance of main product and reactant, a two-phase solvent system was selected by thin layer chromatography, in which the partition coefficient for each component was determined by HPLC. Then a two-phase solvent system consisting of petroleum ether/ethyl acetate/methanol/water (6:4:6:4, v/v) was selected. After acetylation reaction, 20 mg of synthetic mixture was subjected to countercurrent chromatography, yielding 2.1 mg of ferrocene and 8.7 mg of acetyl ferrocene with 96.6% and 97.4% purity. Results indicated that countercurrent chromatography is a very efficient method for separation and purification of target product from synthetic mixtures. GRAPHICAL ABSTRACT\",\"PeriodicalId\":16295,\"journal\":{\"name\":\"Journal of Liquid Chromatography & Related Technologies\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.0000,\"publicationDate\":\"2022-05-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Liquid Chromatography & Related Technologies\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.1080/10826076.2022.2143798\",\"RegionNum\":4,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Liquid Chromatography & Related Technologies","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1080/10826076.2022.2143798","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
Separation of acetylferrocene from synthetic mixtures by countercurrent chromatography
Abstract The main product of acetylation of ferrocene was successfully separated and purified with high purity from synthetic mixtures by countercurrent chromatography using a selected biphasic solvent system. According to distribution performance of main product and reactant, a two-phase solvent system was selected by thin layer chromatography, in which the partition coefficient for each component was determined by HPLC. Then a two-phase solvent system consisting of petroleum ether/ethyl acetate/methanol/water (6:4:6:4, v/v) was selected. After acetylation reaction, 20 mg of synthetic mixture was subjected to countercurrent chromatography, yielding 2.1 mg of ferrocene and 8.7 mg of acetyl ferrocene with 96.6% and 97.4% purity. Results indicated that countercurrent chromatography is a very efficient method for separation and purification of target product from synthetic mixtures. GRAPHICAL ABSTRACT
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The Journal of Liquid Chromatography & Related Technologies is an internationally acclaimed forum for fast publication of critical, peer reviewed manuscripts dealing with analytical, preparative and process scale liquid chromatography and all of its related technologies, including TLC, capillary electrophoresis, capillary electrochromatography, supercritical fluid chromatography and extraction, field-flow technologies, affinity, and much more. New separation methodologies are added when they are developed. Papers dealing with research and development results, as well as critical reviews of important technologies, are published in the Journal.
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