墨西哥一家医院儿科患者造血干细胞移植后随访期间血管内皮生长因子-a水平的监测:一项试点研究

Q4 Medicine Transplantation Reports Pub Date : 2023-09-01 DOI:10.1016/j.tpr.2023.100143
Leticia Itzel Orozco-Ureña , Luis Enrique Juárez Villegas , Felix Gaytan Morales , V. Ivan Castorena , Catalina Cortes Flores , Juan Manuel Colín-Ruiz , Gerardo Aparicio-Ozores , Monica Moreno-Galván
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引用次数: 0

摘要

血管内皮生长因子是参与正常生理、实体瘤和血液恶性肿瘤相关过程的蛋白质;然而,它们在造血干细胞移植(HSCT)中的作用需要进一步研究。为了更好地确定血管内皮生长因子- a (VEGF-A)在造血干细胞移植中的作用和变化,我们对儿童患者造血干细胞移植后早期VEGF-A的表达进行了观察性前瞻性分析。2016年6月至2018年7月期间,37名在墨西哥Federico Gómez儿童医院接受造血干细胞移植的儿童患者被前瞻性纳入本研究。在移植后第0、+7、+14、+21、+28和+35天,从这些患者的静脉血中分离核糖核酸,使用特异性引物和VEGF-A探针进行TaqMan逆转录-聚合酶链反应。采用互补脱氧核糖核酸对照法测定VEGF-A浓度。数据分析采用单因素方差分析和Dunnett事后检验。采用SPSS 25进行统计分析。VEGF-A浓度在第0天与第28天(p = 0.009 95% CI= 0.02-0.24)、第0天与第35天(p = 0.006;95% CI = 0.03 - -0.28),第七天与天+ 35 (p = 0.006;95% CI= 0.03-0.24)。我们观察到,在儿科患者干细胞移植后的早期,VEGF-A浓度显著增加。我们的结果提供了重要的见解,应该被认为是未来儿科HSCT临床试验的基础,包括VEGF-A浓度的监测,蛋白质和体外分析。
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Monitoring vascular endothelial growth factor-a levels during follow-up after hematopoietic stem cell transplantation in pediatric patients at a Mexican hospital: A pilot study

Vascular endothelial growth factors are proteins that participate in processes related to normal physiology, solid tumors and hematologic malignancies; however, their role in hematopoietic stem cell transplantation (HSCT) requires further investigation. To better define the role and changes in vascular endothelial growth factor-A (VEGF-A) in the context of HSCT, we conducted an observational prospective analysis of VEGF-A expression during the early period after HSCT in pediatric patients. Thirty-seven pediatric patients who underwent hematopoietic stem cell transplantation at the Federico Gómez Children's Hospital in Mexico between June 2016 and July 2018 were prospectively enrolled in this study. Ribonucleic acid was isolated from the venous blood of these patents on Days 0, +7, +14, +21, +28, and +35 after transplantation, and TaqMan reverse transcription-polymerase chain reaction was performed using specific primers and a probe for VEGF-A. The concentration of VEGF-A was determined using a complementary deoxyribonucleic acid control. Data were analyzed using one-way ANOVA and Dunnett post hoc tests. Statistical analysis was performed using SPSS version 25. There were significant differences in the concentrations of VEGF-A between Day 0 vs. Day +28 (p = 0.009 95% CI=0.02–0.24), Day 0 vs. Day +35 (p = 0.006; 95% CI=0.03–0.28) and Day 7 vs. Day + 35 (p = 0.006; 95% CI=0.03–0.24) after allogeneic HSCT. We observed significant increases in the VEGF-A concentration during the early period after stem cell transplantation in pediatric patients. Our results provide important insights that should be considered a basis for future clinical trials of pediatric HSCT, including the monitoring of VEGF-A concentrations, proteins and in vitro analysis.

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来源期刊
Transplantation Reports
Transplantation Reports Medicine-Transplantation
CiteScore
0.60
自引率
0.00%
发文量
24
审稿时长
101 days
期刊介绍: To provide to national and regional audiences experiences unique to them or confirming of broader concepts originating in large controlled trials. All aspects of organ, tissue and cell transplantation clinically and experimentally. Transplantation Reports will provide in-depth representation of emerging preclinical, impactful and clinical experiences. -Original basic or clinical science articles that represent initial limited experiences as preliminary reports. -Clinical trials of therapies previously well documented in large trials but now tested in limited, special, ethnic or clinically unique patient populations. -Case studies that confirm prior reports but have occurred in patients displaying unique clinical characteristics such as ethnicities or rarely associated co-morbidities. Transplantation Reports offers these benefits: -Fast and fair peer review -Rapid, article-based publication -Unrivalled visibility and exposure for your research -Immediate, free and permanent access to your paper on Science Direct -Immediately citable using the article DOI
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