黄芩苷通过抑制体内外肝细胞凋亡减轻2型糖尿病肝损伤

IF 4.7 4区医学 Q1 CHEMISTRY, MEDICINAL Chinese Herbal Medicines Pub Date : 2023-10-01 DOI:10.1016/j.chmed.2023.03.007

Xiaoming Fan , Yiyu Wang , Xifan Li , Taiqing Zhong , Chunlan Cheng , Yunfei Zhang

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引用次数: 0

摘要

目的从灯盏花中提取灯盏花苷类主要活性成分。众所周知，黄芩苷具有抗炎、抗氧化的生理功能。本研究检测了黄芩苷对2型糖尿病(T2DM)大鼠肝细胞凋亡的影响。方法SD大鼠(6 ~ 8周，160 ~ 180 g)随机分为对照组、模型组、黄芩苷低、中、高剂量组和罗格列酮阳性组;每组10只SD大鼠(n = 10)。自动生化仪检测血清生化因子变化，苏木精伊红(HE)染色检测肝组织病理变化，组织染色和流量分析仪检测肝组织和细胞凋亡情况，qPCR、Western blot和免疫组化检测肝组织或细胞凋亡相关因子表达。结果黄芩苷能降低空腹血糖、总胆固醇、甘油三酯、低密度脂蛋白水平，提高高密度脂蛋白水平。同时，黄芩苷降低了大鼠谷丙转氨酶(ALT)和天冬氨酸转氨酶(AST)水平，改善了肝功能。此外，黄芩苷还能抑制白细胞介素-1 (IL-1)、白细胞介素-6 (IL-6)和肿瘤坏死因子-α (TNF-α)的分泌，减少肝细胞凋亡。此外，黄芩苷抑制裂解型Caspase-3、Bax和细胞色素C (Cyt-C)的表达，促进Bcl-2的表达。结论黄芩苷可抑制细胞凋亡通路，从而缓解T2DM。

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Scutellarin alleviates liver injury in type 2 diabetic mellitus by suppressing hepatocyte apoptosis in vitro and in vivo

Objective

Scutellarin is a primary active composition come from Erigeron breviscapus. It is well known that scutellarin has anti-inflammatory and antioxidant physiological functions. In this study, we detected the effects of scutellarin on hepatocyte cell apoptosis in type 2 diabetes mellitus (T2DM) rats.

Methods

Sprague Dawley (SD) (6–8 weeks, 160–180 g) rats were randomly divided into six groups: control, model, scutellarin low-dose, medium-dose, high-dose treatment, and rosiglitazone positive groups; with 10 SD rats in each group (n = 10). The changes of biochemical factors in serum were detected by automatic biochemical instrument, the pathological changes of liver tissue were detected by hematoxylin and eosin (HE) staining, the apoptosis of liver tissue and cells was detected by tissue staining and flow analyzer, and the expression of apoptosis-related factors were determined by qPCR, Western blot and immunohistochemistry in liver tissues or cells.

Results

The results showed that scutellarin decreased the levels of fasting blood glucose, total cholesterol, triglyceride, and low-density lipoprotein and increased the levels of high-density lipoprotein. Meanwhile, scutellarin decreased the levels of alanine transaminase (ALT) and aspartate transaminase (AST) and improved liver function. In addition, scutellarin suppressed the secretion of interleukin-1 (IL-1), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) and reduced hepatocyte apoptosis. Furthermore, scutellarin inhibited the expression of cleaved Caspase-3, Bax, and cytochrome C (Cyt-C) and promoted the expression of Bcl-2.