姜黄醇提物对几种巴贝斯虫和马氏菌体外繁殖的影响

M. Rizk, S. El-Sayed, I. Igarashi
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引用次数: 8

摘要

目前市场上的抗血小板药物已被证实对宿主具有毒性和寄生虫耐药性。植物是新药的可能来源。因此,应该采用一种新的策略来寻找既有效又安全的抗血小板药物。在本研究中,我们研究了姜黄(Curcuma longa)甲醇提取物对牛巴贝斯虫(b.b ebovis)、发散巴贝斯虫(b.b edivergens)、caballi巴贝斯虫(b.b ebesia)和马氏巴贝斯虫(Theileria equi)体外生长的影响。用荧光法测定姜黄的体外抑菌效果。采用体外培养的方法,研究了姜黄与乙酸地咪那烯(DA)联合使用对体外抑菌效果的增强作用。姜黄对牛b、发散b、equi和caballi的体外生长均有抑制作用,IC50值分别为0.830±0.078、0.375±0.055、1.405±0.575和0.720±0.090 mg/mL。1 mg/mL姜黄对牛b、0.5 mg/mL发散b、1 mg/mL马t、0.5 mg/mL黄黄对小檗的抑制作用分别为73.43%、80.065%、73.47%、47.375%。当姜黄与DA联用时,其对牛巴贝斯虫和马巴贝斯虫/马伊勒氏菌的体外抑制作用增强。这些发现表明,姜黄的甲醇提取物可能是一种有前途的治疗巴贝斯虫病的药用植物,特别是当与DA联合使用时。
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Effects of Methanolic Extract from Turmeric (Curcuma longa) against the In Vitro Multiplication of Several Babesia Species and Theileria equi
Anti-piroplasm drugs currently on the market have proven toxicity to the host and parasite resistance. Plants are possible sources of novel drugs. Subsequently, a novel strategy should be used to find new anti-piroplasm agents that are both effective and safe. In the present study, we have evaluated the effect of turmeric (Curcuma longa) methanolic extract on the in vitro growth of Babesia (B.) bovis, B. divergens, B. caballi, and Theileria (T.) equi. The in vitro inhibitory effectiveness of turmeric was assessed using a fluorescence test. The enhancement in the in vitro inhibitory efficacy of turmeric when administrated in combination with diminazene aceturate (DA) was investigated using in vitro cultures of different piroplasm parasites. Turmeric reduced the in vitro growth of B. bovis, B. divergens, T. equi, and B. caballi with IC50 values of 0.830 ± 0.078, 0.375 ± 0.055, 1.405 ± 0.575, and 0.720 ± 0.090 mg/mL, respectively. An amount of 1 mg/mL turmeric for B. bovis, 0.5 mg/mL turmeric for B. divergens, 1 mg/mL turmeric for T. equi, and 0.5 mg/mL turmeric for B. caballi exhibited 73.43%, 80.065%, 73.47%, and 47.375% inhibitions in the growth of the parasites, respectively. When turmeric was combined with DA, its in vitro inhibitory impact on bovine Babesia and equine Babesia/Theileria parasites was amplified. These findings show that a methanolic extract of turmeric could be a promising medicinal plant for the treatment of babesiosis, especially when administered in conjunction with DA.
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