山羊初乳对食物中毒病原菌的抑菌活性研究

T. Setyawardani, J. Sumarmono, Heni Risqiati, S. A. Santosa
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引用次数: 1

摘要

本研究旨在研究山羊初乳中植物乳杆菌3CT7和20CT8的抗菌活性。无细胞上清液的抗菌活性使用良好扩散法在几个指标上进行测试:温度、储存时间和pH。在pH 2.0时,两个分离株都记录了抗菌活性;4.0;6.0和8.0,温度分别为0、50和100°C,冷藏0、15、30、45和60天。植物乳杆菌7CT3和植物乳杆菌20CT8与假单胞菌相比具有更大的抑制区。测试无细胞活性旨在研究植物乳杆菌对代谢产物的抑制作用。分离物能够抑制实验中的所有致病菌(百里香、大肠杆菌和金黄色葡萄球菌),从15.83至16.06mm的相似抑制区可以证明这一点。分离物(植物乳杆菌7CT3和20CT8)对百里香表现出抑制特性,金黄色葡萄球菌、假单胞菌属和单核细胞增多性乳杆菌在0、50和100℃下的植物乳杆菌7CT3和植物乳杆菌20CT8在冷藏期间表现出抗菌活性。在pH为2至8的范围内生长的两个分离株都能抑制S。百里香、大肠杆菌、金黄色葡萄球菌和假单胞菌。总的来说,这两个分离株具有广泛的pH、温度和储存时间范围,具有潜在的抗菌活性。
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Antimicrobial Activity of Goat Colostrum Against Bacterial Strains Causing Food Poisoning Diseases
The study was aimed to investigate the antimicrobial activity of bacterial isolates L.plantarum 3CT7 and 20CT8 from goat colostrum. The antimicrobial activity of cell-free supernatant was tested using a well-diffusion method on several indicators: temperature, time of storage, and pH. Antimicrobial activity was recorded in both isolates at pH 2.0; 4.0; 6.0 and 8.0, temperature at 0, 50 and 100 o C, and in cold storage for 0, 15, 30, 45 and 60 days. L.plantarum 7CT3 and L.plantarum 20CT8  have a bigger zone of inhibition than that of Pseudomonas spp. as compared to other bacteria. Testing the cell-free activity was aimed to investigate the metabolite inhibition by L.plantarum . The isolates were capable of inhibiting all pathogenic bacteria in the experiment ( S. thypimurium, E. coli, and S. aureus )  as evidenced from the similar zone of inhibition from 15.83 to 16.06 mm. Isolates ( L. plantarum 7CT3 dan 20CT8) exhibit inhibitory properties against S.thypimurium, S. aureus, Pseudomonas spp. . and L. monocytogenes at 0, 50 and 100 o C. L.plantarum 7CT3 and L.plantarum 20CT8 exhibit antimicrobial activity during cold storage. Both isolates grown in the range of pH from 2 to 8 could inhibit S . thypimurium, E. coli, S. aureus and Pseudomonas spp. In general, the two isolates are the potential antimicrobial activity with broad ranges of pH, temperature and storage time.
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