IL-10基因与COVID-19疾病保护的关联

IF 0.5 4区 医学 Q4 MICROBIOLOGY Jundishapur Journal of Microbiology Pub Date : 2023-08-21 DOI:10.5812/jjm-138241
Najmeh Sheikhi, M. Jamalidoust, Arash Letafati, K. Shahzamani, Anahita Sanaei Dashti, G. Talei
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引用次数: 0

摘要

背景:随着2019冠状病毒病(COVID-19)的大流行,医学界面临着新的挑战,因为疾病的严重程度在很大程度上取决于促炎细胞因子的过度表达,导致内皮功能障碍和器官损伤,尤其是肺部。目的:人们相信突变可能与严重疾病有关。本横断面研究旨在探讨转诊至Namazi医院(设拉子,伊朗)的伊朗儿科患者的COVID-19严重程度与白细胞介素10 (IL-10)基因多态性(rs1800896、rs1800871和rs1800872)之间的相关性。方法:将53例新冠肺炎患儿分为轻/中度组(n = 44)和重度组(n = 9)。研究人员从每位参与研究的患者身上采集了鼻拭子和全血样本。采用实时聚合酶链反应(Real-time polymerase chain reaction, PCR)对SARS-CoV-2进行确证(E, RdRp)和PCR-限制性片段长度多态性(RFLP)对IL-10基因多态性进行基因分型。结果:本研究探讨了IL-10基因多态性与儿童COVID-19严重程度的关系。结果表明,IL10-1082位点GA基因型对严重症状具有保护作用,严重病例均为AA/GA基因型男性。另外两个基因座il -10- 819和IL-10-592与COVID-19严重程度无显著相关性。该研究还表明,呼吸短促是唯一与COVID-19严重程度显著相关的症状,年龄和性别对疾病结果没有影响。结论:轻/中度组患者最常见症状为咳嗽、发热;然而,呼吸急促和咳嗽在严重组中最常见。2019冠状病毒病的严重程度与IL-10 (rs1800872)基因多态性有关,GA基因型具有保护作用。
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Association of IL-10 Gene in Protection Against COVID-19 Disease
Background: The medical community is facing a new challenge with the coronavirus disease 2019 (COVID-19) pandemic, as the severity of the disease is largely determined by the overexpression of proinflammatory cytokines, leading to endothelial dysfunction and organ damage, especially in the lungs. Objectives: It is believed that mutations might be linked to severe illness. This cross-sectional study aimed to explore the correlation between COVID-19 severity in Iranian pediatric patients who were referred to Namazi Hospital (Shiraz, Iran) and interleukin 10 (IL-10) gene polymorphisms (rs1800896, rs1800871, and rs1800872). Methods: The study comprised 53 pediatric patients with COVID-19, who were divided into mild/moderate (n = 44) and severe (n = 9) groups. Nasal swabs and whole blood samples were collected from each patient who participated in the study. Real-time polymerase chain reaction (PCR) for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) confirmation (E, RdRp) and PCR-restriction fragment length polymorphism (RFLP) were used for IL-10 gene polymorphism genotyping. Results: The study investigated the association between IL-10 gene polymorphisms and COVID-19 severity in pediatric patients. The results showed that the GA genotype at the IL10-1082 locus was protective against severe symptoms and that all severe cases were male with the AA/GA genotype. The other two loci, IL10-819 and IL-10-592, did not show any significant association with COVID-19 severity. The study also showed that shortness of breath was the only symptom significantly associated with COVID-19 severity and that age and gender did not affect the disease outcome. Conclusions: The most common symptoms in the mild/moderate group were cough and fever; however, shortness of breath and cough were the most common in the severe group. Coronavirus disease 2019 severity is related to the IL-10 (rs1800872) gene polymorphism, with the GA genotype providing protective effects.
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来源期刊
CiteScore
1.30
自引率
0.00%
发文量
56
审稿时长
6-12 weeks
期刊介绍: Jundishapur Journal of Microbiology, (JJM) is the official scientific Monthly publication of Ahvaz Jundishapur University of Medical Sciences. JJM is dedicated to the publication of manuscripts on topics concerning all aspects of microbiology. The topics include medical, veterinary and environmental microbiology, molecular investigations and infectious diseases. Aspects of immunology and epidemiology of infectious diseases are also considered.
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