日本橙蝇tsuneonbactrocera微卫星标记的研制(双翅目:绢蝇科)

IF 1.3 4区 农林科学 Q2 ENTOMOLOGY Applied Entomology and Zoology Pub Date : 2022-04-29 DOI:10.1007/s13355-022-00783-9
Pattara Opadith, Satsuki Iwamoto, Minoru Narahara, Yoshio Okazaki, Yoshimitsu Higashiura, Jun Otake, Hajime Ono, Norihide Hinomoto
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引用次数: 0

摘要

为了监测和控制日本海啸小蝇(Miyake)(双翅目:绢蝇科),确定其传播能力是非常重要的。因此,为了确定该物种的遗传结构,我们在17个位点上建立了微卫星标记。以日本西部大分县和山口县的废弃果园为研究对象,随机采集80只雌雄成虫,利用微卫星标记进行多态性分析。17个位点的平均等位基因数为2.65个,其中2个位点不存在多态性。期望杂合度在0 ~ 0.663之间,而观察到的杂合度在0 ~ 0.105之间相对较低。零等位基因频率在不同人群中存在差异。此外,在大分县采集的3个幼虫种群均未发现多态性。这些结果表明该物种具有高度的近交。虽然在大分和山口两个群体之间存在显著的遗传分化,但在同一群体中,雌雄之间没有观察到显著的遗传分化。在山口县发现了瓶颈效应,而在大分县没有发现,这与该物种分布扩大的历史一致。这些标记可用于种群区分和扩展该物种的地理历史,为海啸白鱀豚的病虫害管理做出潜在贡献。
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Development of microsatellite markers for the Japanese orange fly, Bactrocera tsuneonis (Diptera: Tephritidae)

To monitor and control the Japanese orange fly Bactrocera tsuneonis (Miyake) (Diptera: Tephritidae), it is important to determine their dispersal ability. Therefore, to determine the genetic structure of this species, we developed microsatellite markers at 17 loci for B. tsuneonis. A total of 80 adult females and males randomly collected from abandoned orchards in the Oita and Yamaguchi Prefectures, in the western part of Japan, were used for polymorphism analysis using the microsatellite markers. The average number of alleles was 2.65 for the 17 loci, in which two loci did not show polymorphism. The expected heterozygosity ranged from 0 to 0.663, although the observed heterozygosity was relatively low between 0 and 0.105. The null allele frequencies varied among populations. Moreover, no polymorphism was found in the three larval populations collected in the Oita Prefecture. These results suggested a high degree of inbreeding for this species. Significant genetic differentiation between females and males of the same population was not observed, although it was observed between the Oita and Yamaguchi populations. A bottleneck effect was detected in Yamaguchi but not in Oita, and this is congruent with the history of an expanding distribution in this species. These markers could be used for population discrimination and to expand the geographic history of this species, potentially contributing to the pest management of B. tsuneonis.

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来源期刊
CiteScore
2.70
自引率
7.70%
发文量
37
审稿时长
6-12 weeks
期刊介绍: Applied Entomology and Zoology publishes articles concerned with applied entomology, applied zoology, agricultural chemicals and pest control in English. Contributions of a basic and fundamental nature may be accepted at the discretion of the Editor. Manuscripts of original research papers, technical notes and reviews are accepted for consideration. No manuscript that has been published elsewhere will be accepted for publication.
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