{"title":"长非编码核糖核酸X无活性特异性转录物/microRNA-29a/磷酸酶和10号染色体通路上张力素同源物缺失在骨髓间充质干细胞成骨分化和绝经后骨质疏松中的作用","authors":"Jian Liu","doi":"10.31901/24566330.2022/22.03.803","DOIUrl":null,"url":null,"abstract":"The researchers aimed to inquire into the roles of long non-coding ribonucleic acid X inactive specific transcript/microRNA-29a/phosphatase and tensin homolog deleted on chromosome ten (lncRNA-XIST/miR-29a/ PTEN) pathway in the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) and postmenopausal osteoporosis (PMOP). In the miR-29a + Lenti-NC group, ALP activity, concentration of alizarin red, and mRNA and protein expressions of Runx2, OPN and OCN significantly increased, whereas the mRNA and protein expressions of LPL, AP-2 and leptin decreased (P<0.05). In contrast with the miR-29a + Lenti-NC group, miR-29a + Lenti-XIST and miR-29a + Lenti-PTEN groups had decreased ALP activity, concentration of alizarin red, and mRNA and protein expressions of Runx2, OPN and OCN, but increased mRNA and protein expressions of LPL, AP-2 and leptin (P<0.05). BMSCs have incremental expressions of lncRNA-XIST and PTEN and a declined expression of miR-29a. LncRNAXIST suppresses the osteogenic differentiation of BMSCs by feat of the miR-29a/PTEN pathway.","PeriodicalId":54956,"journal":{"name":"International Journal of Human Genetics","volume":null,"pages":null},"PeriodicalIF":0.1000,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Roles of Long Non-coding Ribonucleic Acid X Inactive Specific Transcript/microRNA-29a/phosphatase and Tensin Homolog Deleted on Chromosome Ten Pathway in Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells and Postmenopausal Osteoporosis\",\"authors\":\"Jian Liu\",\"doi\":\"10.31901/24566330.2022/22.03.803\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The researchers aimed to inquire into the roles of long non-coding ribonucleic acid X inactive specific transcript/microRNA-29a/phosphatase and tensin homolog deleted on chromosome ten (lncRNA-XIST/miR-29a/ PTEN) pathway in the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) and postmenopausal osteoporosis (PMOP). In the miR-29a + Lenti-NC group, ALP activity, concentration of alizarin red, and mRNA and protein expressions of Runx2, OPN and OCN significantly increased, whereas the mRNA and protein expressions of LPL, AP-2 and leptin decreased (P<0.05). In contrast with the miR-29a + Lenti-NC group, miR-29a + Lenti-XIST and miR-29a + Lenti-PTEN groups had decreased ALP activity, concentration of alizarin red, and mRNA and protein expressions of Runx2, OPN and OCN, but increased mRNA and protein expressions of LPL, AP-2 and leptin (P<0.05). BMSCs have incremental expressions of lncRNA-XIST and PTEN and a declined expression of miR-29a. LncRNAXIST suppresses the osteogenic differentiation of BMSCs by feat of the miR-29a/PTEN pathway.\",\"PeriodicalId\":54956,\"journal\":{\"name\":\"International Journal of Human Genetics\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.1000,\"publicationDate\":\"2022-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Human Genetics\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.31901/24566330.2022/22.03.803\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"GENETICS & HEREDITY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Human Genetics","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.31901/24566330.2022/22.03.803","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}
Roles of Long Non-coding Ribonucleic Acid X Inactive Specific Transcript/microRNA-29a/phosphatase and Tensin Homolog Deleted on Chromosome Ten Pathway in Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells and Postmenopausal Osteoporosis
The researchers aimed to inquire into the roles of long non-coding ribonucleic acid X inactive specific transcript/microRNA-29a/phosphatase and tensin homolog deleted on chromosome ten (lncRNA-XIST/miR-29a/ PTEN) pathway in the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) and postmenopausal osteoporosis (PMOP). In the miR-29a + Lenti-NC group, ALP activity, concentration of alizarin red, and mRNA and protein expressions of Runx2, OPN and OCN significantly increased, whereas the mRNA and protein expressions of LPL, AP-2 and leptin decreased (P<0.05). In contrast with the miR-29a + Lenti-NC group, miR-29a + Lenti-XIST and miR-29a + Lenti-PTEN groups had decreased ALP activity, concentration of alizarin red, and mRNA and protein expressions of Runx2, OPN and OCN, but increased mRNA and protein expressions of LPL, AP-2 and leptin (P<0.05). BMSCs have incremental expressions of lncRNA-XIST and PTEN and a declined expression of miR-29a. LncRNAXIST suppresses the osteogenic differentiation of BMSCs by feat of the miR-29a/PTEN pathway.
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