牵牛花提取物在彩色植物切片制备中的应用

S. Wahyuni, Siti Zaenab, Windi Kurniasari, Ayu Iriani, Dewinta Hapsari Maharani
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引用次数: 0

摘要

背景:制备需要着色以澄清或锐化各种组织部分,尤其是细胞。从长远来看,在制剂中使用合成染料对生物和环境的影响较小。本研究旨在从牵牛花中在最佳温度和pH下获得天然染料,并探讨用牵牛花提取物着色制剂的可行性。方法:提取过程采用浸渍法,不同温度变化为400℃、500℃、600℃、700℃、800℃,不同pH值为3、4、5、6。制备过程使用不同的染料浓度变化。使用的浓度为50%、60%、70%、80%、90%、100%。数据分析技术使用描述性和百分比分析方法。结果:牵牛花提取物(Ipomoea purpurea(L.)Roth)能对辣椒(Capsicum frutescens L.)茎上的真皮组织、empulur、皮层和运输束进行染色。
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Application of Morning Glory Flower (Ipomea purpurea) Extract for Colouring Plant Section Preparation
Background: Preparing requires colouring to clarify or sharpen various tissue parts, especially cells. The use of synthetic dyes in preparations in the long term can less impact both living things and the environment. The study aims to obtain natural dyes from morning glory flowers at optimal temperatures and pH and find out the feasibility of preparations coloured using Morning Glory flower extract. Method: The extraction process uses a maceration method with different temperature variations of 400C, 500C, 600C, 700C, 800C and different pH i.e. 3, 4, 5, 6. The preparation process uses different dye concentration variations. The concentrations used are 50%, 60%, 70%, 80%, 90%, 100%. Data analysis techniques use descriptive and percentage analysis methods. Results: Morning glory flower extract (Ipomoea purpurea (L.) Roth) can colour dermal tissue, empulur, cortex and transport beams on the stems of cayenne pepper plants (Capsicum frutescens L.). Conclusion: Natural dyes of morning glory flowers can be used as a learning medium with a 74% eligibility rate for preparing with morning glory flower dyes.
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