{"title":"基于质谱的蛋白质组学分析平台","authors":"Y. Ishihama","doi":"10.15583/jpchrom.2019.023","DOIUrl":null,"url":null,"abstract":"Mass spectrometry-based proteomics platforms have been widely used as ‘proteome sequencers’ to characterize the proteomes of a wide range of organisms. The work-flow generally involves multiple steps of sample preparation, peptide purification/concentration/pre-fractionation, and nanoLC/MS/MS measurement. This review focuses on our contributions to the technical development of current proteomics platforms, and includes a consideration of the limitations of these systems, together with the prospects for developing superior new-generation proteome sequencers.","PeriodicalId":91226,"journal":{"name":"Chromatography (Basel)","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2019-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.15583/jpchrom.2019.023","citationCount":"4","resultStr":"{\"title\":\"Analytical Platforms for Mass Spectrometry-Based Proteomics\",\"authors\":\"Y. Ishihama\",\"doi\":\"10.15583/jpchrom.2019.023\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Mass spectrometry-based proteomics platforms have been widely used as ‘proteome sequencers’ to characterize the proteomes of a wide range of organisms. The work-flow generally involves multiple steps of sample preparation, peptide purification/concentration/pre-fractionation, and nanoLC/MS/MS measurement. This review focuses on our contributions to the technical development of current proteomics platforms, and includes a consideration of the limitations of these systems, together with the prospects for developing superior new-generation proteome sequencers.\",\"PeriodicalId\":91226,\"journal\":{\"name\":\"Chromatography (Basel)\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2019-10-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.15583/jpchrom.2019.023\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Chromatography (Basel)\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.15583/jpchrom.2019.023\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chromatography (Basel)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.15583/jpchrom.2019.023","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Analytical Platforms for Mass Spectrometry-Based Proteomics
Mass spectrometry-based proteomics platforms have been widely used as ‘proteome sequencers’ to characterize the proteomes of a wide range of organisms. The work-flow generally involves multiple steps of sample preparation, peptide purification/concentration/pre-fractionation, and nanoLC/MS/MS measurement. This review focuses on our contributions to the technical development of current proteomics platforms, and includes a consideration of the limitations of these systems, together with the prospects for developing superior new-generation proteome sequencers.
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