光谱法分析复制品ACEH微量拷贝中CAFEIN

Sabarni Sabarni, N. Nurhayati
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引用次数: 4

摘要

亚齐除了被称为“Serambi Mekah”的城市外,还被称为是“千家咖啡店”的城市。其中一道吸引消费者的咖啡菜是khop咖啡(读作:kopi khop),因为它是用倒置的玻璃盘盛的。khop咖啡是一种典型的咖啡饮料,来自西亚齐摄政区的Meulaboh,它是通过将锅上装满粗咖啡粉的杯子倒过来供应的。因此,有必要对khop咖啡中独特的味觉决定化合物进行专门的分析研究,以将亚齐人的当地智慧提升到科学领域。采用薄层色谱法和紫外-可见分光光度法测定了咖啡粉粒径变化引起的khop咖啡中咖啡因化合物的浓度。这项研究使用了四种直径变化的咖啡样品,即原始、精细、中等和粗糙。当调酒师提供这四个样品(90°C)时,根据相同的条件,用沸水提取四个样品,然后用氯仿溶剂进行液-液萃取。通过使用TLC然后使用UV-Vis光谱仪分析干提取物。结果表明,原咖啡、细咖啡、中咖啡和粗咖啡的浓度分别为57.85%;17.61%;1.29%;0.83%。
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ANALISIS KADAR KAFEIN DALAM MINUMAN KOPI KHOP ACEH DENGAN METODE SPEKTROSKOPIK
In addition of being dubbed as the city of "Serambi Mekah", Aceh is also famous as the city of "Thousand Coffee Shops". One of coffee dish that attracts consumers' is khop coffee (read: kopi khop) because it is served with a glass dish upside down. The khop coffee, a typical coffee drink from Meulaboh, West Aceh Regency, is served by reversing the glass that has been filled with coarse coffee powder on top of the pan. Therefore, it is deemed necessary to conduct a special study on the analysis of unique taste-determining compounds in khop coffee to elevate the local wisdom of the Acehnese to the scientific region. The aim of this study was to determine the concentration of caffeine compounds of khop coffee due to the modification of coffee powder diameter by using the thin layer chromatography (TLC) method and UV-Vis spectrophotometry. This study used four types of samples with diameter variations coffee, namely original, fine, medium, and coarse. The four samples were extracted with boiling hot water according to the same conditions when the bartender served it (90 °C) followed by liquid-liquid extraction using chloroform solvent. Dry extract was analyzed by using TLC followed by a UV-Vis spectrometer. The results showed that the concentrations of original, fine, medium, and coarse coffee were respectively 57.85%; 17.61%; 1.29%; and 0.83%.
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