应用表型和分子方法检测伊朗巴波尔医学科学大学附属医院临床标本中大肠杆菌、克雷伯菌和肠杆菌属以及产AmpC的肠杆菌科的患病率

Z. Shahandeh, F. Sadighian, N. Kalantrai
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引用次数: 2

摘要

背景与目的:产生ampc的细菌是治疗革兰氏阴性菌引起的传染病的严重威胁。由于没有可靠的诊断方法来检测这些细菌,因此这些细菌的实际流行情况尚不清楚。因此,本研究通过表型和分子方法确定临床样本中大肠埃希菌、克雷伯菌和肠杆菌产生AmpC的频率。材料与方法:本研究对2018年不同临床样本分离的163种肠杆菌科细菌进行检测。采用头孢西丁纸片法和纸片扩散法对产生pAmpC的可疑菌株进行了鉴定。采用三种验证性表型方法鉴定pAmpC的产生,并使用分子方法搜索所有细菌的blaDHA, blaFOX, blaMOX基因。与存在blaDHAgene相比,获得表型试验的特异性和敏感性。结果:163株细菌中有80株(49.1%)对FOX耐药,21株(12.9%)携带blaDHA基因。在携带该基因的细菌中,5株(6%)对FOX敏感。49个(61.3%)fox耐药菌在染色体和/或质粒表型试验中呈阳性。AmpC盘法特异性和灵敏度最高,分别为90.8%和42.7%。结论:表型法在鉴别真阴性方面更成功(特异性更高)。此外,对头孢西丁的敏感性并不是不产生AmpC酶的标准。因此,建议在全国范围内开展AmpC产菌基因鉴定监测工作。
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Prevalence Escherichia coli, Klebsiella and Enterobacter Species and AmpC-producing Enterobacteriaceae in Clinical Specimens of Hospitals Affiliated to Babol University of Medical Sciences, Iran using Phenotypic and Molecular Methods
Background and Aim: AmpC-producing bacteria are a severe threat to treating infectious diseases caused by gram-negative bacteria. The actual prevalence of these bacteria is not clearly determined as there is no reliable diagnostic method available to detect them. Therefore, this study was performed to determine the frequency of Escherichia coli, Klebsiella, and Enterobacter species producing AmpC among clinical samples by phenotypic and molecular methods. Materials and Methods: In this study, 163 bacteria of Enterobacteriaceae species isolated from different clinical samples in 2018 were examined. Suspected isolates of producing pAmpC were identified using cefoxitin disk (FOX) and disk diffusion method. Three confirmatory phenotypic methods were performed to identify pAmpC production, and blaDHA, blaFOX, blaMOX genes were searched using a molecular method for all bacteria. Specificity and sensitivity of phenotypic tests were obtained compared to the presence of blaDHAgene. Results: Of 163 bacteria, 80 (49.1%) isolates were resistant to FOX, and 21 (12.9%) carried the blaDHA gene. Among the bacteria carrying the gene, 5 (6%) isolates were sensitive to FOX. 49 (61.3%) FOX-resistance bacteria were positive in one of the chromosomal and/or plasmid phenotypic tests. The highest specificity and sensitivity were observed in the AmpC disk (90.8%) and CAM (42.7%) methods, respectively. Conclusion: It seems phenotypic methods are more successful in distinguishing true negatives (higher specificity). Also, sensitivity to cefoxitin is not a criterion for not producing the enzyme AmpC. For this reason, it is recommended that national monitoring be performed to identify the genes of AmpC producing bacteria.
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来源期刊
Iranian Journal of Medical Microbiology
Iranian Journal of Medical Microbiology Medicine-Infectious Diseases
CiteScore
1.60
自引率
0.00%
发文量
70
审稿时长
8 weeks
期刊最新文献
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