{"title":"RAPD-PCR技术检测桃蝇、带状双峰虫和雌性γ射线辐射后的遗传变异","authors":"R. Sayed, N. Zahran, A. Hamza","doi":"10.3157/021.130.0304","DOIUrl":null,"url":null,"abstract":"ABSTRACT. Bactrocera zonata (Saunders) is a destructive polyphagous insect that infests various fruits and vegetables worldwide. Nowadays, radiation technology is widely used as a safe tool for controlling it. Therefore, this study was designed to explore the effect of gamma radiation on adult females of B. zonata. Full-grown pupae were irradiated with female sterilizing and substerilizing doses of gamma irradiation. Exposure to gamma radiation-induced alterations in DNA patterns of adult females, the appearance of some extra bands and the deficiency of others in the RAPD-PCR amplification patterns of irradiated females when compared with unirradiated females.","PeriodicalId":11719,"journal":{"name":"Entomological News","volume":null,"pages":null},"PeriodicalIF":0.4000,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Genetic Variation in Peach Fruit Fly, Bactrocera zonata, Females Irradiated with Gamma Radiation as Revealed by RAPD-PCR Technique\",\"authors\":\"R. Sayed, N. Zahran, A. Hamza\",\"doi\":\"10.3157/021.130.0304\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"ABSTRACT. Bactrocera zonata (Saunders) is a destructive polyphagous insect that infests various fruits and vegetables worldwide. Nowadays, radiation technology is widely used as a safe tool for controlling it. Therefore, this study was designed to explore the effect of gamma radiation on adult females of B. zonata. Full-grown pupae were irradiated with female sterilizing and substerilizing doses of gamma irradiation. Exposure to gamma radiation-induced alterations in DNA patterns of adult females, the appearance of some extra bands and the deficiency of others in the RAPD-PCR amplification patterns of irradiated females when compared with unirradiated females.\",\"PeriodicalId\":11719,\"journal\":{\"name\":\"Entomological News\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.4000,\"publicationDate\":\"2022-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Entomological News\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.3157/021.130.0304\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"ENTOMOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Entomological News","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.3157/021.130.0304","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
Genetic Variation in Peach Fruit Fly, Bactrocera zonata, Females Irradiated with Gamma Radiation as Revealed by RAPD-PCR Technique
ABSTRACT. Bactrocera zonata (Saunders) is a destructive polyphagous insect that infests various fruits and vegetables worldwide. Nowadays, radiation technology is widely used as a safe tool for controlling it. Therefore, this study was designed to explore the effect of gamma radiation on adult females of B. zonata. Full-grown pupae were irradiated with female sterilizing and substerilizing doses of gamma irradiation. Exposure to gamma radiation-induced alterations in DNA patterns of adult females, the appearance of some extra bands and the deficiency of others in the RAPD-PCR amplification patterns of irradiated females when compared with unirradiated females.
期刊介绍：
Contributions are accepted on any of the aspects of systematics and taxonomy of insects. This includes: taxonomic revisions, phylogenetics, biogeography and faunistics, and descriptive morphology of insects. Taxonomic papers describing single species are acceptable if such a study is sufficiently justified, however, comprehensive studies that provide a comparative, integrated study of insect systematics and taxonomy will get priority.
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