荧光假单胞菌对含羞草幼苗生长的影响

Daniceli Barcellos, Vanessa Gisele Dambros, Y. Kondo, Andressa Vasconcelos Flores, Jussara Cristina Stinghen, G. Botelho
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引用次数: 0

摘要

含羞草(Mimosa scabrella Benth)是巴西南部土生土长的豆科植物,具有巨大的商业利用潜力。由于其生长速度快,适应性强,根瘤形成能力强,可用于恢复退化地区。另一种降低种苗生产施肥成本的方法是促进植物生长的根瘤菌。假单胞菌的荧光基团普遍存在于几种植物的根际。本研究的目的是评估荧光假单胞菌分离物对苞片植物幼苗生长的刺激作用。种子接种三种分离菌株(CBSAL14、CBSAL18和CBSAL05),基因鉴定为假单胞菌sp.将其在50 mL的Luria Bertani培养基中培养48 h,然后将种子在悬浮液中浸泡30 min。随后,它们被播种在含有灭菌的商业基质的管中,并在温室中保存5个月。从播种后61 d开始,按月测定苗高和茎粗。与对照相比,CBSAL14和CBSAL18分离物可促进接种个体的苗高生长,提示其作为植物生长促进剂的潜在应用价值。
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Influence of fluorescent Pseudomonas on the growth of Mimosa scabrella seedlings
Bracantiga (Mimosa scabrella Benth) is a native leguminous specie from the south of Brazil, with great potential for commercial use. It can be used to recover degraded areas due to their fast growth, high adaptability and root nodule formation capacity. One alternative to reduce fertilization costs of seedlings production, are the plant growth promoting rhizobacteria. The fluorescent group of Pseudomonas is common at several plant rhizosphere. The objective of this work was to assess the growth stimulus of fluorescent Pseudomonas isolates at bracatinga seedlings. The seeds were inoculated with three bacterial isolates (CBSAL14, CBSAL18 and CBSAL05), genetically identified as Pseudomonas sp. They were previously grown in 50 mL of Luria Bertani medium for 48 h and then the seeds were submerged for 30 min in the suspensions. Subsequently, they were sown in tubes containing sterilized commercial substrate and kept in greenhouse for five months. From 61 days after sowing, seedling height and stem diameter were measured monthly. The CBSAL14 and CBSAL18 isolates promoted promoted increase in growth of seedlings height of inoculated individuals compared to control, suggesting their potential use as plant growth promoters.
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