pGEM-T质粒在大肠杆菌JM109中转化的温度和时间优化

Q4 Medicine Gaceta Medica de Caracas Pub Date : 2023-08-23 DOI:10.47307/gmc.2023.131.s4.8

Fusvita Merdekawati

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引用次数: 0

摘要

简介：广泛使用的转化方法是热休克法，它受到热休克处理时间和温度的影响。然而，对pGEM-T质粒在大肠杆菌JM109中转化的最佳温度缺乏研究。本研究采用热休克法确定了pGEM-T在大肠杆菌JM109中转化的最佳温度和时间。

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Temperature and Time Optimization of pGEM-T Plasmid Transformation in Escherichia coli JM109

Introduction: The widely used method for transformation was the heat shock method, which was influenced by the time and temperature of heat shock treatment. However, there was a lack of research on the optimal temperature for pGEM-T plasmid transformation in Escherichia coli JM109. This study uses the heat shock method to determine the optimum temperature and time for pGEM-T transformation in Escherichia coli JM109.

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来源期刊

Gaceta Medica de Caracas Medicine-Medicine (all)

CiteScore

0.30

自引率

0.00%

发文量

160

期刊介绍： Gaceta Médica de Caracas Órgano de la Academia Nacional de Medicina y del Congreso Venezolano de Ciencias Médicas Salud Fundada el 13 de marzo de 1893 Trimestral GMC