HTLV-1感染期间免疫基因表达谱失调

IF 0.8 Q4 GENETICS & HEREDITY Meta Gene Pub Date : 2021-12-01 DOI:10.1016/j.mgene.2021.100944
Masoud Keikha , Mohammad Ali-Hassanzadeh , Ramin Bagheri , Mohsen Karbalaei
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引用次数: 0

摘要

人类嗜T淋巴病毒1型(HTLV-1)是成人T细胞白血病/淋巴瘤(ATLL)和HTLV-1相关脊髓病/热带痉挛性截瘫(HAM/TSP)的主要病因。本研究的目的是利用微阵列数据集评估可能参与ATLL发病机制的免疫基因失调。方法从GEO数据库中获取ATLL病例GSE19080的基因表达谱。其次,采用MetaQC对数据的质量和可靠性进行评估,并对各组的表达数据进行affy包归一化处理。随后,应用R包MetaDE进行差异表达基因(deg)分析。利用STRING数据库,构建了轮毂基因的蛋白-蛋白相互作用网络(PPIN)。最后,利用在线服务器(包括STRING、enrichment和KEGG pathway)对结果进行基因富集和解释。结果65例显著性hub deg分为正常健康者、无症状携带者和ATLL患者3组。轮毂deg之间的PPIN分析采用STRING进行。富集分析显示,中枢deg参与细胞凋亡、T细胞增殖、Ras信号、MAPK信号、NF-κB信号、整合素信号、P53信号、血管生成、组织侵袭和DNA损伤等多种通路。结论HTLV-1可能通过促进细胞增殖引起炎症反应。在HTLV-1感染过程中,免疫基因如IL-10、TGF-β、JAK、BCL2等的失调导致HTLV-1感染的CD4+ T细胞永生化,最终发展为ATLL。
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Dysregulation of immune gene expression profiles during HTLV-1 infection

Background

Human T-lymphotropic virus type 1 (HTLV-1) is the main cause of adult T cell leukemia/lymphoma (ATLL) and HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). The aim of this study was to evaluate the dysregulation of immune genes that may be involved in the pathogenesis of ATLL using microarray datasets.

Method

The gene expression profiles of ATLL cases (GSE19080) were obtained from GEO database. Next, the quality and reliability of data were evaluated by MetaQC, and the expression data in each group were normalized by affy package. Subsequently, the R package MetaDE was applied for the analysis of differentially expressed genes (DEGs). Using STRING database, protein-protein interaction network (PPIN) was constructed for hub DEGs. Finally, online servers including STRING, Enrichr, and KEGG pathway were applied for gene enrichment and interpretation of the results.

Results

65 significant hub DEGs were divided in three groups, normal health, asymptomatic carrier and ATLL patients. The PPIN analysis between hub DEGs was carried out by STRING. Enrichment analysis revealed that the hub DEGs were involved in various pathways such as apoptosis, proliferation of T cell, Ras signaling, MAPK signaling, NF-κB signaling, integrin signaling, P53 signaling, angiogenesis, tissue invasion, and DNA damage process.

Conclusion

According to the present study, HTLV-1 appears to cause inflammation by enhancing cell proliferation. During the HTLV-1 infection, dysregulation of immune genes such as IL-10, TGF-β, JAK, BCL2, etc. result in immortalization of HTLV-1-infected CD4+ T cells, and eventually progression to ATLL.

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来源期刊
Meta Gene
Meta Gene Biochemistry, Genetics and Molecular Biology-Genetics
CiteScore
1.10
自引率
0.00%
发文量
20
期刊介绍: Meta Gene publishes meta-analysis, polymorphism and population study papers that are relevant to both human and non-human species. Examples include but are not limited to: (Relevant to human specimens): 1Meta-Analysis Papers - statistical reviews of the published literature of human genetic variation (typically linked to medical conditionals and/or congenital diseases) 2Genome Wide Association Studies (GWAS) - examination of large patient cohorts to identify common genetic factors that influence health and disease 3Human Genetics Papers - original studies describing new data on genetic variation in smaller patient populations 4Genetic Case Reports - short communications describing novel and in formative genetic mutations or chromosomal aberrations (e.g., probands) in very small demographic groups (e.g., family or unique ethnic group). (Relevant to non-human specimens): 1Small Genome Papers - Analysis of genetic variation in organelle genomes (e.g., mitochondrial DNA) 2Microbiota Papers - Analysis of microbiological variation through analysis of DNA sequencing in different biological environments 3Ecological Diversity Papers - Geographical distribution of genetic diversity of zoological or botanical species.
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