新冠肺炎分子诊断试验中基于Toehold开关方法的最新进展

IF 1.3 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Journal of Nucleic Acids Pub Date : 2022-05-09 DOI:10.1155/2022/7130061
Almando Geraldi, N. N. T. Puspaningsih, Fatiha Khairunnisa
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引用次数: 1

摘要

在2019冠状病毒病(COVID-19)大流行期间,需要大量诊断检测才能获得具有代表性的结果。这些结果可以帮助设计和实施有效的政策,以防止严重急性呼吸综合征冠状病毒2 (SARS-CoV-2)的传播。使用当前的金标准方法,即实时定量PCR (RT-qPCR)进行诊断具有挑战性,特别是在训练有素的人员和卫生相关基础设施有限的地区。基于支点开关的诊断系统具有单次检测成本低、分析速度快、灵敏度高、特异性强等优点,是一种很有前途的新方法。此外,该系统可应用于纸质平台,简化了资源匮乏环境下的分配和利用。本文综述了基于脚点开关的诊断设备作为检测SARS-CoV-2的最新方法的发展。
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Update on the Development of Toehold Switch-Based Approach for Molecular Diagnostic Tests of COVID-19
A high volume of diagnostic tests is needed during the coronavirus disease 2019 (COVID-19) pandemic to obtain representative results. These results can help to design and implement effective policies to prevent the spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Diagnosis using current gold standard methods, i.e., real-time quantitative PCR (RT-qPCR), is challenging, especially in areas with limited trained personnel and health-related infrastructure. The toehold switch-based diagnostic system is a promising alternative method for detecting SARS-CoV-2 that has advantages such as inexpensive cost per testing, rapid, and highly sensitive and specific analysis. Moreover, the system can be applied to paper-based platforms, simplifying the distribution and utilization in low-resource settings. This review provides insight into the development of toehold switch-based diagnostic devices as the most recent methods for detecting SARS-CoV-2.
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来源期刊
Journal of Nucleic Acids
Journal of Nucleic Acids BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
3.10
自引率
21.70%
发文量
5
审稿时长
12 weeks
期刊最新文献
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