CpG 684:小鼠COVID-19灭活疫苗的有效佐剂

IF 4.7 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Applied Bio Materials Pub Date : 2023-05-01 Epub Date: 2023-06-01 DOI:10.2217/fvl-2022-0172

Jiandong Liu, Tianle Cang, Congli Jiang, Kelei Li, Siyuan Liu, Haixin Wang, Meirong Wang, Yan Chen, Yan Shao, Jiankai Liu

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引用次数: 0

摘要

目的：应用CpG 684作为新冠肺炎灭活疫苗的佐剂，检测小鼠的体液和细胞免疫反应。材料与方法：我们使用10和20µg CpG 684作为新冠肺炎灭活疫苗的佐剂免疫小鼠。ELISA法检测血清中IgG、IgG1、IgG2a、IgG2b和IgM结合抗体。ELISPOT检测IFN-γ细胞因子。结果：CpG 684改善了刺突特异性IgG和IgM亚型结合抗体，并提高了针对原型、德尔塔和贝塔毒株的中和抗体滴度。CpG 684还改善了细胞免疫反应。结论：CpG 684是新冠肺炎灭活疫苗的有效佐剂。

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CpG 684: an effective adjuvant for the inactivated COVID-19 vaccine in mice.

Aim: This study used CpG 684 as adjuvant of inactivated COVID-19 vaccine to detect a humoral and cellular immune response in mice.

Materials & methods: We used 10 and 20 µg CpG 684 as adjuvants of an inactivated COVID-19 vaccine to immunize mice. IgG, IgG1, IgG2a, IgG2b and IgM binding antibodies were detected in serum by ELISA. The IFN-γ cytokine was detected by ELISPOT.

Results: CpG 684 improved spike-specific IgG and IgM subtype binding antibodies and increased the neutralizing antibody titer against prototype, Delta and Beta strains. CpG 684 also improved cellular immune response.

Conclusion: CpG 684 is an effective adjuvant for inactivated COVID-19 vaccine.

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来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464

期刊介绍： ACS Applied Bio Materials is an interdisciplinary journal publishing original research covering all aspects of biomaterials and biointerfaces including and beyond the traditional biosensing, biomedical and therapeutic applications. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important bio applications. The journal is specifically interested in work that addresses the relationship between structure and function and assesses the stability and degradation of materials under relevant environmental and biological conditions.