城市饮用水中幽门螺杆菌基因(CagA和VacA)的检测

IF 1.3 Q4 ENVIRONMENTAL SCIENCES Environmental Health Engineering and Management Journal Pub Date : 2022-09-10 DOI:10.34172/ehem.2022.31
F. Gholami-Borujeni, H. Rahimi, Seyed Mehdi Sajjadi
{"title":"城市饮用水中幽门螺杆菌基因(CagA和VacA)的检测","authors":"F. Gholami-Borujeni, H. Rahimi, Seyed Mehdi Sajjadi","doi":"10.34172/ehem.2022.31","DOIUrl":null,"url":null,"abstract":"Background: Helicobacter pylori is classified as a carcinogen, and it is also the most common cause of chronic bacterial infection and peptic ulcers. Approximately 45% of people are infected with the bacterium. Methods: In this study, the H. pylori genes, CagA and VacA, were investigated in drinking water, using 100 samples (50 samples from the municipal water supply and 50 samples from the effluent of household water treatment devices). DNA was extracted from colonies with a positive heterotrophic plate count (HPC) for use in molecular testing and microbial identification. The polymerase chain reaction (PCR) was used to identify H. pylori. Results: The study showed that 24% of urban water samples (12% above the World Health Organization [WHO] standards for safe drinking water) and 18% of home water treatment-device samples (4% above the WHO standards) were HPC-positive. The H. pylori genes, CagA and VacA, were identified in 2% of the samples from household water treatment devices and 8% of the municipal water supply samples. Conclusion: The study findings show that H. pylori may be transmitted in drinking water. However, there is currently no strong evidence that the bacteria can survive after the disinfection process in the water supply system. Therefore, the health risks of this bacterium in drinking water are still unknown.","PeriodicalId":51877,"journal":{"name":"Environmental Health Engineering and Management Journal","volume":null,"pages":null},"PeriodicalIF":1.3000,"publicationDate":"2022-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Detection of Helicobacter pylori genes (CagA and VacA) in municipal drinking water\",\"authors\":\"F. Gholami-Borujeni, H. Rahimi, Seyed Mehdi Sajjadi\",\"doi\":\"10.34172/ehem.2022.31\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Background: Helicobacter pylori is classified as a carcinogen, and it is also the most common cause of chronic bacterial infection and peptic ulcers. Approximately 45% of people are infected with the bacterium. Methods: In this study, the H. pylori genes, CagA and VacA, were investigated in drinking water, using 100 samples (50 samples from the municipal water supply and 50 samples from the effluent of household water treatment devices). DNA was extracted from colonies with a positive heterotrophic plate count (HPC) for use in molecular testing and microbial identification. The polymerase chain reaction (PCR) was used to identify H. pylori. Results: The study showed that 24% of urban water samples (12% above the World Health Organization [WHO] standards for safe drinking water) and 18% of home water treatment-device samples (4% above the WHO standards) were HPC-positive. The H. pylori genes, CagA and VacA, were identified in 2% of the samples from household water treatment devices and 8% of the municipal water supply samples. Conclusion: The study findings show that H. pylori may be transmitted in drinking water. However, there is currently no strong evidence that the bacteria can survive after the disinfection process in the water supply system. Therefore, the health risks of this bacterium in drinking water are still unknown.\",\"PeriodicalId\":51877,\"journal\":{\"name\":\"Environmental Health Engineering and Management Journal\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.3000,\"publicationDate\":\"2022-09-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Environmental Health Engineering and Management Journal\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.34172/ehem.2022.31\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"ENVIRONMENTAL SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Environmental Health Engineering and Management Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.34172/ehem.2022.31","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
引用次数: 1

摘要

背景:幽门螺杆菌被列为致癌物质,也是慢性细菌感染和消化性溃疡最常见的原因。大约45%的人感染了这种细菌。方法:在本研究中,使用100个样本(50个来自城市供水的样本和50个来自家庭水处理装置出水的样本)对饮用水中的幽门螺杆菌基因CagA和VacA进行了调查。从具有阳性异养平板计数(HPC)的菌落中提取DNA,用于分子测试和微生物鉴定。采用聚合酶链式反应(PCR)对幽门螺杆菌进行了鉴定。结果:研究表明,24%的城市水样(高于世界卫生组织安全饮用水标准的12%)和18%的家庭水处理设备样品(高于世界卫生组织标准的4%)为HPC-阳性。幽门螺杆菌基因CagA和VacA在2%的家庭水处理设备样本和8%的城市供水样本中被鉴定。结论:研究结果表明幽门螺杆菌可能在饮用水中传播。然而,目前还没有强有力的证据表明,在供水系统中消毒后,细菌可以存活。因此,这种细菌在饮用水中的健康风险仍然未知。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Detection of Helicobacter pylori genes (CagA and VacA) in municipal drinking water
Background: Helicobacter pylori is classified as a carcinogen, and it is also the most common cause of chronic bacterial infection and peptic ulcers. Approximately 45% of people are infected with the bacterium. Methods: In this study, the H. pylori genes, CagA and VacA, were investigated in drinking water, using 100 samples (50 samples from the municipal water supply and 50 samples from the effluent of household water treatment devices). DNA was extracted from colonies with a positive heterotrophic plate count (HPC) for use in molecular testing and microbial identification. The polymerase chain reaction (PCR) was used to identify H. pylori. Results: The study showed that 24% of urban water samples (12% above the World Health Organization [WHO] standards for safe drinking water) and 18% of home water treatment-device samples (4% above the WHO standards) were HPC-positive. The H. pylori genes, CagA and VacA, were identified in 2% of the samples from household water treatment devices and 8% of the municipal water supply samples. Conclusion: The study findings show that H. pylori may be transmitted in drinking water. However, there is currently no strong evidence that the bacteria can survive after the disinfection process in the water supply system. Therefore, the health risks of this bacterium in drinking water are still unknown.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
2.40
自引率
37.50%
发文量
17
审稿时长
12 weeks
期刊最新文献
Two phases of online food delivery app users’ behavior in Greater Jakarta during the second year of the COVID-19 pandemic: Perceptions of food safety and hygiene Feasibility of natural wastewater treatment systems and life cycle assessment (LCA) for aquatic systems Modeling the concentration of suspended particles by fuzzy inference system (FIS) and adaptive neuro-fuzzy inference system (ANFIS) techniques: A case study in the metro stations Plant inoculation with Piriformospora indica fungus and additive effects of organic and inorganic Zn fertilize on decreasing the Cd concentration of the plants cultivated in the Cd-polluted soil Quantitative assessment of health, safety, and environment (HSE) resilience based on the Delphi method and analytic hierarchy process (AHP) in municipal solid waste management system: A case study in Tehran
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1